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Validation Data Gallery

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  • Western Blot (WB) analysis of rat brain tissue using TRIM2 Monoclonal antibody (67342-1-Ig)

    WB analysis of rat brain using 67342-1-Ig (same clone as 67342-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 67342-1-Ig (TRIM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67342-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse cerebellum tissue using TRIM2 Monoclonal antibody (67342-1-Ig)

    WB analysis of mouse cerebellum using 67342-1-Ig (same clone as 67342-1-PBS)

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 67342-1-Ig (TRIM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67342-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using TRIM2 Monoclonal antibody (67342-1-Ig)

    WB analysis of pig brain using 67342-1-Ig (same clone as 67342-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67342-1-Ig (TRIM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67342-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TRIM2 Monoclonal antibody (67342-1-Ig)

    IHC staining of mouse brain using 67342-1-Ig (same clone as 67342-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67342-1-Ig (TRIM2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67342-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TRIM2 Monoclonal antibody (67342-1-Ig)

    IHC staining of mouse brain using 67342-1-Ig (same clone as 67342-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67342-1-Ig (TRIM2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67342-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using TRIM2 Monoclonal antibody (67342-1-Ig)

    IHC staining of human gliomas using 67342-1-Ig (same clone as 67342-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67342-1-Ig (TRIM2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67342-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using TRIM2 Monoclonal antibody (67342-1-Ig)

    IHC staining of human gliomas using 67342-1-Ig (same clone as 67342-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67342-1-Ig (TRIM2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67342-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using TRIM2 Monoclonal antibody (67342-1-Ig)

    IF Staining of mouse brain using 67342-1-Ig (same clone as 67342-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TRIM2 antibody (67342-1-Ig, Clone: 1H9C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67342-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using TRIM2 Monoclonal antibody (67342-1-Ig)

    IF Staining of mouse brain using 67342-1-Ig (same clone as 67342-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TRIM2 antibody (67342-1-Ig, Clone: 1H9C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67342-1-PBS in a different storage buffer formulation.

TRIM2 Monoclonal antibody, PBS Only

TRIM2 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 67342-1-PBS
Clone No.1H9C2

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

KIAA0517, RING finger protein 86, RNF86, TRIM2, tripartite motif containing 2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67342-1-PBS targets TRIM2 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag14637

Product name: Recombinant human TRIM2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-300 aa of BC011052

Sequence: MASEGTNIPSPVVRQIDKQFLICSICLERYKNPKVLPCLHTFCERCLQNYIPAHSLTLSCPVCRQTSILPEKGVAALQNNFFITNLMDVLQRTPGSNAEESSILETVTAVAAGKPLSCPNHDGNVMEFYCQSCETAMCRECTEGEHAEHPTVPLKDVVEQHKASLQVQLDAVNKRLPEIDSALQFISEIIHQLTNQKASIVDDIHSTFDELQKTLNVRKSVLLMELEVNYGLKHKVLQSQLDTLLQGQESIKSCSNFTAQALNHGTETEVLLVKKQMSEKLNELADQDFPLHPRENDQLD

相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 2
Calculated molecular weight 744 aa, 82 kDa
Observed molecular weight 70-85 kDa
GenBank accession numberBC011052
Gene Symbol TRIM2
Gene ID (NCBI) 23321
RRIDAB_2882600
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ9C040
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Tripartite motif-containing 2(TRIM2) is an E3 ubiquitin ligase which directs proteasome-mediated degradation of target proteins by the ubiquitination of NEFL and phosphorylation of BCL2L11. This protein contains an N-terminal RING domain, followed by a B-box-2 domain, a coiled-coil region, and 6 C-terminal NHL repeats. TRIM2 involves in the apoptotic response to ischemia via directing degradation of BIM protein, thus has a role in neuroprotection.

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