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  • KD/KO Validated

TRIM2 Polyclonal antibody

TRIM2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 20356-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

E3 ubiquitin-protein ligase TRIM2, EC:2.3.2.27, KIAA0517, RING-type E3 ubiquitin transferase TRIM2, RNF86

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inmouse brain tissue, mouse small intestine tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, human kidney tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20356-1-AP targets TRIM2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIM2 fusion protein Ag14208 相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 2
Calculated molecular weight 744 aa, 82 kDa
Observed molecular weight 70-85 kDa
GenBank accession numberBC011052
Gene Symbol TRIM2
Gene ID (NCBI) 23321
RRIDAB_10734319
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9C040
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tripartite motif-containing 2(TRIM2) is an E3 ubiquitin ligase which directs proteasome-mediated degradation of target proteins by the ubiquitination of NEFL and phosphorylation of BCL2L11. This protein contains an N-terminal RING domain, followed by a B-box-2 domain, a coiled-coil region, and 6 C-terminal NHL repeats. TRIM2 involves in the apoptotic response to ischemia via directing degradation of BIM protein, thus has a role in neuroprotection.

Protocols

Product Specific Protocols
WB protocol for TRIM2 antibody 20356-1-APDownload protocol
IHC protocol for TRIM2 antibody 20356-1-APDownload protocol
IF protocol for TRIM2 antibody 20356-1-APDownload protocol
IP protocol for TRIM2 antibody 20356-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP,IF

Nat Commun

Divergent self-association properties of paralogous proteins TRIM2 and TRIM3 regulate their E3 ligase activity

Authors - Diego Esposito
humanWB,IF

J Transl Med

Stress-induced epinephrine promotes epithelial-to-mesenchymal transition and stemness of CRC through the CEBPB/TRIM2/P53 axis.

Authors - Zili Zhou
humanWB

Cancer Cell Int

TRIM2 directly deubiquitinates and stabilizes Snail1 protein, mediating proliferation and metastasis of lung adenocarcinoma.

Authors - Zhaoxian Lin
  • KD Validated
humanWB,IP,IF

FEBS J

TRIM2 promotes metabolic adaptation to glutamine deprivation via enhancement of CPT1A activity

Authors - Kaimin Liao
humanWB,IHC

Cell Mol Life Sci

E3 ubiquitin ligase TRIM2 identified as a novel suppressor of CYP11B2 and aldosterone production

Authors - Liang Chen
  • KD Validated
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