Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human testis tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 10 publications below |
| IHC | See 4 publications below |
| IF | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
24403-1-AP targets TRIM16 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21406 Product name: Recombinant human TRIM16 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-349 aa of BC015674 Sequence: EMQFGELLAAVRKAQANVMLFLEEKEQAALSQANGIKAHLEYRSAEMEKSKQELERMAAISNTVQFLEEYCKFKNTEDITFPSVYVGLKDKLSGIRKVITESTVHLIQLLENYKKKLQEFSKEEEYDIRTQVSAVVQRKYWTSKPEPSTREQFLQYAYDITFDPDTAHKYLRLQEENRKVTNTTPWEHPYPDLPSRFLHWRQVLSQQSLYLHRYYFEVEIFGAGTYVGLTCKGIDRKGEERNSCISGNNFSWSLQWNGKEFTAWYSDMETPLKAGPFRRLGVYIDFPGGILSFYGVEYDTMTLVHKFACKFSEPVYAAFWLSKKENAIRIVDLGEEPEKPAPSLVGTAP 相同性解析による交差性が予測される生物種 |
| Full Name | tripartite motif-containing 16 |
| Calculated molecular weight | 64 kDa |
| Observed molecular weight | 64 kDa |
| GenBank accession number | BC015674 |
| Gene Symbol | TRIM16 |
| Gene ID (NCBI) | 10626 |
| RRID | AB_2879525 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95361 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TRIM16 (tripartite motif-containing protein 16) is an E3 ubiquitin ligase belonging to the tripartite motif (TRIM) protein family, mediates the ubiquitination of its target proteins and tags them for degradation. TRIM16 controls ubiquitination of damaged compartments and regulates the core autophagy regulators ULK1, Beclin 1, and ATG16L1, which confer localized autophagic sequestration of damaged lysosomes (PMID: 27693506, 22629402). TRIM16 overexpression also enhances retinoidinduced differentiation, reduces neuroblastoma cell growth, migration, and significantly reduces tumorigenicity in vivo (PMID: 36504902).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TRIM16 antibody 24403-1-AP | Download protocol |
| IHC protocol for TRIM16 antibody 24403-1-AP | Download protocol |
| IP protocol for TRIM16 antibody 24403-1-AP | Download protocol |
| WB protocol for TRIM16 antibody 24403-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol TRIM16 Promotes Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Modulating CHIP-Mediated Degradation of RUNX2.
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J Genet Genomics RASSF6-TRIM16 axis promotes cell proliferation, migration and invasion in esophageal squamous cell carcinoma. | ||
Exp Cell Res TRIM16 protects human periodontal ligament stem cells from oxidative stress-induced damage via activation of PICOT. | ||
Exp Cell Res TRIM16 overexpression inhibits the metastasis of colorectal cancer through mediating Snail degradation.
| ||
Cell Prolif G3BP1 coordinates lysophagy activity to protect against compression-induced cell ferroptosis during intervertebral disc degeneration | ||
Mol Carcinog TRIM16 E121D variant affects the risk and prognosis of hepatocellular carcinoma by modulating the Wnt/β-catenin pathway
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