Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, human testis tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human testis tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 8 publications below |
| WB | See 15 publications below |
| IHC | See 4 publications below |
| IF | See 3 publications below |
| IP | See 2 publications below |
| CoIP | See 2 publications below |
Product Information
24403-1-AP targets TRIM16 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21406 Product name: Recombinant human TRIM16 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-349 aa of BC015674 Sequence: EMQFGELLAAVRKAQANVMLFLEEKEQAALSQANGIKAHLEYRSAEMEKSKQELERMAAISNTVQFLEEYCKFKNTEDITFPSVYVGLKDKLSGIRKVITESTVHLIQLLENYKKKLQEFSKEEEYDIRTQVSAVVQRKYWTSKPEPSTREQFLQYAYDITFDPDTAHKYLRLQEENRKVTNTTPWEHPYPDLPSRFLHWRQVLSQQSLYLHRYYFEVEIFGAGTYVGLTCKGIDRKGEERNSCISGNNFSWSLQWNGKEFTAWYSDMETPLKAGPFRRLGVYIDFPGGILSFYGVEYDTMTLVHKFACKFSEPVYAAFWLSKKENAIRIVDLGEEPEKPAPSLVGTAP 相同性解析による交差性が予測される生物種 |
| Full Name | tripartite motif-containing 16 |
| Calculated molecular weight | 64 kDa |
| Observed molecular weight | 64 kDa |
| GenBank accession number | BC015674 |
| Gene Symbol | TRIM16 |
| Gene ID (NCBI) | 10626 |
| RRID | AB_2879525 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95361 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TRIM16 (tripartite motif-containing protein 16) is an E3 ubiquitin ligase belonging to the tripartite motif (TRIM) protein family, mediates the ubiquitination of its target proteins and tags them for degradation. TRIM16 controls ubiquitination of damaged compartments and regulates the core autophagy regulators ULK1, Beclin 1, and ATG16L1, which confer localized autophagic sequestration of damaged lysosomes (PMID: 27693506, 22629402). TRIM16 overexpression also enhances retinoidinduced differentiation, reduces neuroblastoma cell growth, migration, and significantly reduces tumorigenicity in vivo (PMID: 36504902).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TRIM16 antibody 24403-1-AP | Download protocol |
| IHC protocol for TRIM16 antibody 24403-1-AP | Download protocol |
| IP protocol for TRIM16 antibody 24403-1-AP | Download protocol |
| WB protocol for TRIM16 antibody 24403-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Autophagy TRIM16 mediates secretory autophagy in head and neck cancer-associated fibroblasts | ||
Adv Sci (Weinh) ZDHHC18-Mediated Palmitoylation of ORF3a Promotes SARS-CoV-2 Pathogenesis by Antagonizing TRIM16-Mediated Ubiquitination and Proteasomal Degradation.
| ||
Adv Sci (Weinh) Morusin Alleviates Aortic Valve Calcification by Inhibiting Valve Interstitial Cell Senescence Through Ccnd1/Trim25/Nrf2 Axis | ||
Adv Sci (Weinh) Sanggenol L Enhances Temozolomide Drug Sensitivity by Inhibiting Mitophagy and Inducing Apoptosis Through the Regulation of the TRIM16-OPTN Axis in Glioblastoma
| ||
Cell Death Dis Autophagy-dependent secretion of ENO1 mediates chemoresistance of glioblastoma and tumor microenvironment remodeling. | ||
Cell Commun Signal TGF-β2 increases eHSP90α secretion via upregulating secretory autophagy pathway.
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