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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TRIM15 Recombinant antibody (83582-5-RR)

    IF Staining of HeLa using 83582-5-RR (same clone as 83582-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TRIM15 antibody (83582-5-RR, Clone: 240565C7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83582-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83582-5-RR

    Affinity and Kinetic Characterization of 83582-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83582-5-RR against Human TRIM15 were performed. The affinity constant is 0.686 nM.

TRIM15 Recombinant monoclonal antibody, PBS Only

TRIM15 Uni-rAb® Recombinant Antibody for IF/ICC, ELISA
Cat No. 83582-5-PBS
Clone No.240565C7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Synonyms

RNF93, TRIM, RING finger protein 93, EC:2.3.2.27, E3 ubiquitin-protein ligase TRIM15

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83582-5-PBS targets TRIM15 in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag4533

Product name: Recombinant human TRIM15 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-270 aa of BC038585

Sequence: MPATPSLKVVHELPACTLCAGPLEDAVTIPCGHTFCRLCLPALSQMGAQSSGKILLCPLCQEEEQAETPMAPVPLGPLGETYCEEHGEKIYFFCENDAEFLCVFCREGPTHQAHTVGFLDEAIQPYRDRLRSRLEALSTERDEIEDVKCQEDQKLQVLLTQIESKKHQVETAFERLQQELEQQRCLLLARLRELEQQIWKERDEYITKVSEEVTRLGAQVKELEEKCQQPASELLQDVRVNQSRCEMKTFVSPEAISPDLVKKIRDFHRK

相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 15
Calculated molecular weight 465 aa, 52 kDa
GenBank accession numberBC038585
Gene Symbol TRIM15
Gene ID (NCBI) 89870
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9C019
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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