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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRIM14 Polyclonal antibody (15742-1-AP)

    WB analysis using 15742-1-AP (same clone as 15742-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15742-1-AP (TRIM14 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15742-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using TRIM14 Polyclonal antibody (15742-1-AP)

    WB analysis of L02 using 15742-1-AP (same clone as 15742-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 15742-1-AP (TRIM14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15742-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using TRIM14 Polyclonal antibody (15742-1-AP)

    IHC staining of human liver using 15742-1-AP (same clone as 15742-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 15742-1-AP (TRIM14 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15742-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using TRIM14 Polyclonal antibody (15742-1-AP)

    IHC staining of human liver using 15742-1-AP (same clone as 15742-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 15742-1-AP (TRIM14 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15742-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using TRIM14 Polyclonal antibody (15742-1-AP)

    IHC staining of human liver using 15742-1-AP (same clone as 15742-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 15742-1-AP (TRIM14 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15742-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using TRIM14 Polyclonal antibody (15742-1-AP)

    IHC staining of human liver using 15742-1-AP (same clone as 15742-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 15742-1-AP (TRIM14 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15742-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TRIM14 Polyclonal antibody (15742-1-AP)

    IF Staining of HeLa using 15742-1-AP (same clone as 15742-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TRIM14 antibody (15742-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15742-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TRIM14 Polyclonal antibody, PBS Only

TRIM14 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 15742-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15742-1-PBS targets TRIM14 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag8158

Product name: Recombinant human TRIM14 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-346 aa of BC006333

Sequence: MAGAATGSRTPGRSELVEGCGWRCPEHGDRVAELFCRRCRRCVCALCPVLGAHRGHPVGLALEAAVHVQKLSQECLKQLAIKKQQHIDNITQIEDATEKLKANAESSKTWLKGKFTELRLLLDEEEALAKKFIDKNTQLTLQVYREQADSCREQLDIMNDLSNRVWSISQEPDPVQRLQAYTATEQEMQQQMSLGELCHPVPLSFEPVKSFFKGLVEAVESTLQTPLDIRLKESINCQLSDPSSTKPGTLLKTSPSPERSLLLKYARTPTLDPDTMHARLRLSADRLTVRCGLLGSLGPVPVLRFDALWQVLARDCFATGRHYWEVDVQEAGAGWWVGAAYASLRR

相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 14
Calculated molecular weight 442 aa, 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC006333
Gene Symbol TRIM14
Gene ID (NCBI) 9830
RRIDAB_2209324
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14142
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Tripartite motif-containing 14(TRIM14) belongs to the TRIM protein family that contains 3 zinc-binding domains, a RING, a B-box type1 and a B-box type2 and plays key roles in cellular proliferation, differentiation, development, transformation, and immune response mechanisms . TRIM4 inhibits the transcriptional activity of SPI1 in a dose-dependent manner. It may participate in the early stages of differentiation of embryonic stem cells, leading to the formation of embryoid bodies.

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