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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RFP2 Polyclonal antibody

RFP2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22021-1-AP

Synonyms

CAR, DLEU5, LEU5, Leukemia associated protein 5, Putative tumor suppressor RFP2, Ret finger protein 2, RFP2, RING finger protein 77, RNF77, TRIM13, tripartite motif containing 13

Validation Data Gallery

Applications:
  • WB analysis of Jurkat using 22021-1-AP

    WB analysis of Jurkat using 22021-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22021-1-AP (RFP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 22021-1-AP

    IP experiment of Jurkat using 22021-1-AP

    IP result of anti-RFP2 (IP:22021-1-AP, 4ug; Detection:22021-1-AP 1:1000) with Jurkat cells lysate 3200ug.

  • IHC staining of human breast cancer using 22021-1-AP

    IHC staining of human breast cancer using 22021-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22021-1-AP (RFP2 antibody at dilution of 1:50.

  • IHC staining of human breast cancer using 22021-1-AP

    IHC staining of human breast cancer using 22021-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22021-1-AP (RFP2 antibody at dilution of 1:50.

Tested Applications

Positive WB detected inJurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman breast cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

The immunogen of 22021-1-AP is RFP2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RFP2 fusion protein Ag16805 相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 13
Calculated molecular weight 407 aa, 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC003579
Gene symbol TRIM13
Gene ID (NCBI) 10206
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Publications

SpeciesApplicationTitle
humanIF

J Biol Chem

Comparative proximity biotinylation implicates the small GTPase RAB18 in sterol mobilization and biosynthesis

Authors - Robert S Kiss
View Article