Validation Data Gallery
Tested Applications
| Positive WB detected in | L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 10 publications below |
| WB | See 38 publications below |
| IHC | See 15 publications below |
| IF | See 6 publications below |
| IP | See 2 publications below |
| CoIP | See 2 publications below |
Product Information
13300-1-AP targets TRIB3 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4085 Product name: Recombinant human TRIB3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-358 aa of BC027484 Sequence: MRATPLAAPAGSLSRKKRLELDDNLDTERPVQKRARSGPQPRLPPCLLPLSPPTAPDRATAVATASRLGPYVLLEPEEGGRAYRALHCPTGTEYTCKVYPVQEALAVLEPYARLPPHKHVARPTEVLAGTQLLYAFFTRTHGDMHSLVRSRHRIPEPEAAVLFRQMATALAHCHQHGLVLRDLKLCRFVFADRERKKLVLENLEDSCVLTGPDDSLWDKHACPAYVGPEILSSRASYSGKAADVWSLGVALFTMLAGHYPFQDSEPVLLFGKIRRGAYALPAGLSAPARCLVRCLLRREPAERLTATGILLHPWLRQDPMPLAPTRSHLWEAAQVVPDGLGLDEAREEEGDREVVLYG 相同性解析による交差性が予測される生物種 |
| Full Name | tribbles homolog 3 (Drosophila) |
| Calculated molecular weight | 358 aa, 40 kDa |
| Observed molecular weight | 40-45 kDa |
| GenBank accession number | BC027484 |
| Gene Symbol | TRIB3 |
| Gene ID (NCBI) | 57761 |
| RRID | AB_10641988 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96RU7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TRIB3 is a pseudokinase molecule that affects a number of cellular functions. TRIB3 has been reported to be highly activated in the presence of a variety of stressors, including the deprivation of neurotrophic factors, hypoxia, and ER stress.TRIB3 protein is associated with a good prognosis in human breast cancer patients, possibly due to the fact that TRIB3 is involved in hypoxia tolerance.(PMID: 21704407). TRIB3 protein predicted molecular weights is approximately 39~45 kDa.(PMID: 31191318, PMID: 24414038, PMID: 31578236).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TRIB3 antibody 13300-1-AP | Download protocol |
| WB protocol for TRIB3 antibody 13300-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Clin Mol Hepatol MET promotes hepatocellular carcinoma development through the promotion of TRIB3-mediated FOXO1 degradation
| ||
Autophagy ER stress elicits non-canonical CASP8 (caspase 8) activation on autophagosomal membranes to induce apoptosis | ||
Autophagy Cannabidiol inhibits human glioma by induction of lethal mitophagy through activating TRPV4.
| ||
Cell Death Dis RNA N6-methyladenosine demethylase FTO promotes diabetic wound healing through TRIB3-mediated autophagy in an m6A-YTHDF2-dependent manner | ||
Cell Death Dis TRIB3 promotes malignancy of head and neck squamous cell carcinoma via inhibiting ferroptosis |