Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21226-1-AP targets TRHDE in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15681 Product name: Recombinant human TRHDE protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 675-1024 aa of BC150181 Sequence: KGSWLLGNINQTGYFRVNYDLRNWRLLIDQLIRNHEVLSVSNRAGLIDDAFSLARAGYLPQNIPLEIIRYLSEEKDFLPWHAASRALYPLDKLLDRMENYNIFNEYILKQVATTYIKLGWPKNNFNGSLVQASYQHEELRREVIMLACSFGNKHCHQQASTLISDWISSNRNRIPLNVRDIVYCTGVSLLDEDVWEFIWMKFHSTTAVSEKKILLEALTCSDDRNLLNRLLNLSLNSEVVLDQDAIDVIIHVARNPHGRDLAWKFFRDKWKILNTRYGEALFMNSKLISGVTEFLNTEGELKELKNFMKNYDGVAAASFSRAVETVEANVRWKMLYQDELFQWLGKALRH 相同性解析による交差性が予測される生物種 |
| Full Name | thyrotropin-releasing hormone degrading enzyme |
| Calculated molecular weight | 1024 aa, 117 kDa |
| Observed molecular weight | 122 kDa |
| GenBank accession number | BC150181 |
| Gene Symbol | TRHDE |
| Gene ID (NCBI) | 29953 |
| RRID | AB_3742022 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UKU6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Thyrotropin-releasing hormone-degrading ectoenzyme (TRHDE) belongs to the peptidase M1 family. TRHDE expression is detected at relatively late phases of brain development. TRHDE functions to inactivate thyrotropin-releasing hormone (PMID: 30518145, PMID: 32457627).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRHDE antibody 21226-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |