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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRHDE Polyclonal antibody (21226-1-AP)

    WB analysis using 21226-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21226-1-AP (TRHDE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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TRHDE Polyclonal antibody

TRHDE Polyclonal Antibody for WB, ELISA
Cat No. 21226-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

TRH degrading ectoenzyme, TRH DE, Thyroliberinase, Pyroglutamyl peptidase II, PGPEP2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21226-1-AP targets TRHDE in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRHDE fusion protein Ag15681 相同性解析による交差性が予測される生物種
Full Name thyrotropin-releasing hormone degrading enzyme
Calculated molecular weight 1024 aa, 117 kDa
Observed molecular weight122 kDa
GenBank accession numberBC150181
Gene Symbol TRHDE
Gene ID (NCBI) 29953
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UKU6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Thyrotropin-releasing hormone-degrading ectoenzyme (TRHDE) belongs to the peptidase M1 family. TRHDE expression is detected at relatively late phases of brain development. TRHDE functions to inactivate thyrotropin-releasing hormone (PMID: 30518145, PMID: 32457627).

Protocols

Product Specific Protocols
WB protocol for TRHDE antibody 21226-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols