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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRH Polyclonal antibody (25332-1-AP)

    WB analysis using 25332-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25332-1-AP (TRH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using TRH Polyclonal antibody (25332-1-AP)

    IHC staining of human brain using 25332-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25332-1-AP (TRH antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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TRH Polyclonal antibody

TRH Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25332-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Prothyroliberin, Pro-thyrotropin-releasing hormone, Protirelin, Thyrotropin-releasing hormone, TRF

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, BxPC-3 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25332-1-AP targets TRH in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRH fusion protein Ag12623 相同性解析による交差性が予測される生物種
Full Name thyrotropin-releasing hormone
Calculated molecular weight 242 aa, 27 kDa
Observed molecular weight27-33 kDa
GenBank accession numberBC074889
Gene Symbol TRH
Gene ID (NCBI) 7200
RRIDAB_3085786
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP20396
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRH, Thyrotropin-releasing hormone, as a component of the hypothalamic-pituitary-thyroid axis, controls the secretion of thyroid-stimulating hormone (TSH) and is involved in thyroid hormone synthesis regulation. It also operates as modulator of hair growth and promotes hair-shaft elongation, prolongs the hair cycle growth phase (anagen) and antagonizes its termination (catagen) by TGFB2. It stimulates proliferation and inhibits apoptosis of hair matrix keratinocytes. TRH is expressed in hypothalamus and the hair follicle epithelium (PMID: 19825978).

Protocols

Product Specific Protocols
WB protocol for TRH antibody 25332-1-APDownload protocol
IHC protocol for TRH antibody 25332-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols