• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TREM2 Monoclonal antibody

TREM2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1F4H10

Cat no : 68723-1-Ig

Synonyms

TREM 2, TREM2, Trem2a, Trem2b, Trem2c

Validation Data Gallery

Applications:
  • WB analysis using 68723-1-Ig

    WB analysis using 68723-1-Ig

    Non-treated and PMA treated THP-1 cellls were subjected to SDS PAGE followed by western blot with 68723-1-Ig (TREM2 antibody) at dilution of 1:5000 incubated at room temperature for 1 hours.

  • IHC staining of mouse brain using 68723-1-Ig

    IHC staining of mouse brain using 68723-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68723-1-Ig (TREM2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 68723-1-Ig

    IHC staining of mouse brain using 68723-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68723-1-Ig (TREM2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inTHP-1 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Sample-dependent, check data in validation data gallery

Product Information

68723-1-Ig targets TREM2 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TREM2 fusion protein Ag6843 相同性解析による交差性が予測される生物種
Full Name triggering receptor expressed on myeloid cells 2
Calculated molecular weight 222 aa, 25 kDa
Observed molecular weight26-32 kDa
GenBank accession numberBC032362
Gene symbol TREM2
Gene ID (NCBI) 54209
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TREM2 (triggering receptor expressed on myeloid cells 2) is a cell surface receptor belongs to TREM family that is expressed on osteoclast, dendritic cells, macrophages, nature killers, neutrophils and microglia (PMID: 19302484). TREM2 is localized predominantly in the Golgi complex, but also shuttles to and from the cell surface in endocytic and exocytic vesicles (PMID: 16675145). TREM2 associates with DAP12 to initiate the intracellular signalling cascade via an immunoreceptor tyrosine-based activation motif (ITAM) domain and tyrosine-kinases (PMID: 23977213). TREM2 has a calculated molecular mass of ~25kDa, but apparent molecular mass of ~40kDa (PMID: 24078628). In addition, TREM2 has a soluble form termed as sTREM-2 with molecular weights ranging between about 24kDa and 40kDa (PMID: 18790823). Loss-of-function mutations in either TREM2 or DAP12 can cause Nasu-Hakola disease (PMID: 19302484).

Protocols

Product Specific Protocols
WB protocol for TREM2 antibody 68723-1-IgDownload protocol
IHC protocol for TREM2 antibody 68723-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols