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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TREK1/KCNK2 Polyclonal antibody (26807-1-AP)

    WB analysis using 26807-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26807-1-AP (TREK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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TREK1/KCNK2 Polyclonal antibody

TREK1/KCNK2 Polyclonal Antibody for WB, ELISA
Cat No. 26807-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

hTREK 1c, hTREK 1e, K2p2.1, KCNK2, TPKC1, TREK, TREK 1, TREK 1 K(+) channel subunit, TREK1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, SH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26807-1-AP targets TREK1/KCNK2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TREK1/KCNK2 fusion protein Ag25209 相同性解析による交差性が予測される生物種
Full Name potassium channel, subfamily K, member 2
Calculated molecular weight 411 aa, 46 kDa
Observed molecular weight40-50 kDa
GenBank accession numberBC101693
Gene Symbol TREK1
Gene ID (NCBI) 3776
RRIDAB_3085905
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95069
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The tandem of pore domains in a weak inward rectifying K+ channel (TWIK1, K2P1.1, or KCNK1) and TWIK-related K+channel 1 (TREK1, K2P 2.1, or KCNK2) are members of the two pore domain potassium (K2P) channel family, consisting of 15 channels that regulate the stabilization of resting membrane potential and cellular excitability by wielding background K+ leakage currents (PMID:12580339). TREK1/KCNK2 is sensitive to a wide range of physical and chemical cues. Its main role is to maintain the resting potential of the cell and whilst highly expressed in the nervous system, TREK1/KCNK2 is also expressed in the kidney, heart, lung and smooth muscle cells (PMID:31031627).

Protocols

Product Specific Protocols
WB protocol for TREK1/KCNK2 antibody 26807-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols