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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRBV5-4 Polyclonal antibody

TRBV5-4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13524-1-AP

Synonyms

TCRBV5S4, TCRBV5S6A3N2T, TRBV5 4, TRBV54, TRBV5-4

Validation Data Gallery

Applications:
  • WB analysis of U-937 using 13524-1-AP

    WB analysis of U-937 using 13524-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 13524-1-AP (TRBV5-4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • WB analysis of U-937 using 13524-1-AP

    WB analysis of U-937 using 13524-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 13524-1-AP (TRBV5-4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inU-937 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Sample-dependent, check data in validation data gallery

Product Information

13524-1-AP targets TRBV5-4 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRBV5-4 fusion protein Ag4337 相同性解析による交差性が予測される生物種
Full Name T cell receptor beta variable 5-4
Calculated molecular weight 308 aa, 34 kDa
Observed molecular weight 30-34 kDa
GenBank accession numberBC028083
Gene symbol TRBV5-4
Gene ID (NCBI) 28611
RRIDAB_1167555
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TRBV5-4 antibody 13524-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols