Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse thymus tissue, Jurkat cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 1 publications below |
Product Information
13279-1-AP targets TRAT1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4023 Product name: Recombinant human TRAT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 30-186 aa of BC025713 Sequence: ISHYVEKQRQDKMYSYSSDHTRVDEYYIEDTPIYGNLDDMISEPMDENCYEQMKARPEKSVNKMQEATPSAQATNETQMCYASLDHSVKGKRRKPRKQNTHFSDKDGDEQLHAIDASVSKTTLVDSFSPESQAVEENIHDDPIRLFGLIRAKREPIN 相同性解析による交差性が予測される生物種 |
| Full Name | T cell receptor associated transmembrane adaptor 1 |
| Calculated molecular weight | 186 aa, 21 kDa |
| Observed molecular weight | 29 kDa |
| GenBank accession number | BC025713 |
| Gene Symbol | TRAT1 |
| Gene ID (NCBI) | 50852 |
| RRID | AB_10643524 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6PIZ9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRAT1 antibody 13279-1-AP | Download protocol |
| IHC protocol for TRAT1 antibody 13279-1-AP | Download protocol |
| IP protocol for TRAT1 antibody 13279-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |