×
Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using TRAPPC4 Polyclonal antibody (12074-1-AP)

    WB analysis using 12074-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12074-1-AP (TRAPPC4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using TRAPPC4 Polyclonal antibody (12074-1-AP)

    WB analysis of MCF-7 using 12074-1-AP

    WB result of TRAPPC4 antibody (12074-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAPPC4 transfected MCF-7 cells.

  • Western Blot (WB) analysis of mouse testis tissue using TRAPPC4 Polyclonal antibody (12074-1-AP)

    WB analysis of mouse testis using 12074-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12074-1-AP (TRAPPC4 antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

  • Immunoprecipitation (IP) experiment of HL-60 cells using TRAPPC4 Polyclonal antibody (12074-1-AP)

    IP experiment of HL-60 using 12074-1-AP

    IP result of anti-TRAPPC4 (IP:12074-1-AP, 4ug; Detection:12074-1-AP 1:800) with HL-60 cells lysate 1720 ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TRAPPC4 Polyclonal antibody (12074-1-AP)

    IHC staining of human colon cancer using 12074-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12074-1-AP (TRAPPC4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TRAPPC4 Polyclonal antibody (12074-1-AP)

    IHC staining of human colon cancer using 12074-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12074-1-AP (TRAPPC4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using TRAPPC4 Polyclonal antibody (12074-1-AP)

    IHC staining of mouse liver using 12074-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12074-1-AP (TRAPPC4 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TRAPPC4 Polyclonal antibody (12074-1-AP)

    IF Staining of HeLa using 12074-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRAPPC4 antibody (12074-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO Validated

TRAPPC4 Polyclonal antibody

TRAPPC4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 12074-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

CGI 104, CGI-104, HSPC172, PTD009, SBDN

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHL-60 cells, MCF-7 cells, mouse testis tissue, rat testis tissue
Positive IP detected inHL-60 cells
Positive IHC detected inhuman colon cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12074-1-AP targets TRAPPC4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2737

Product name: Recombinant human TRAPPC4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-219 aa of BC010866

Sequence: MAIFSVYVVNKAGGLIYQLDSYAPRAEAEKTFSYPLDLLLKLHDERVLVAFGQRDGIRVGHAVLAINGMDVNGRYTADGKEVLEYLGNPANYPVSIRFGRPRLTSNEKLMLASMFHSLFAIGSQLSPEQGSSGIEMLETDTFKLHCYQTLTGIKFVVLADPRQAGIDSLLRKIYEIYSDFALKNPFYSLEMPIRCELFDQNLKLALEVAEKAGTFGPGS

相同性解析による交差性が予測される生物種
Full Name trafficking protein particle complex 4
Calculated molecular weight 219 aa, 24 kDa
Observed molecular weight 24-27 kDa
GenBank accession numberBC010866
Gene Symbol TRAPPC4
Gene ID (NCBI) 51399
RRIDAB_2208147
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y296
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transport protein particle (TRAPP, also known as trafficking protein particle), a multimeric guanine nucleotide-exchange factor, regulates multiple membrane trafficking pathways (PMID: 20966969). TRAPPC4, also known as synbindin, is a core component of the TRAPP complexes and one of the essential subunits for guanine nucleotide exchange factor activity for Rab1 GTPase (PMID: 31794024). Deficiencies in vesicular transport mediated by TRAPPC4 have been associated with severe syndromic intellectual disability (PMID: 31794024).

Protocols

Product Specific Protocols
IF protocol for TRAPPC4 antibody 12074-1-APDownload protocol
IHC protocol for TRAPPC4 antibody 12074-1-APDownload protocol
IP protocol for TRAPPC4 antibody 12074-1-APDownload protocol
WB protocol for TRAPPC4 antibody 12074-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Res

TRAPPC4 Promotes GPX4 Stability to Drive Ferroptosis Resistance and Tumor Progression in Head and Neck Squamous Cell Carcinoma

Authors - Zhao Ding
View Article
||
New chat

Able™

正在加载，请稍候...