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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRAPPC12 Polyclonal antibody (24555-1-AP)

    WB analysis using 24555-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 24555-1-AP (TRAPPC12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using TRAPPC12 Polyclonal antibody (24555-1-AP)

    WB analysis of K-562 using 24555-1-AP

    WB result of TRAPPC12 antibody (24555-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAPPC12 transfected K-562 cells.

  • Immunoprecipitation (IP) experiment of K-562 cells using TRAPPC12 Polyclonal antibody (24555-1-AP)

    IP experiment of K-562 using 24555-1-AP

    IP result of anti-TRAPPC12 (IP:24555-1-AP, 4ug; Detection:24555-1-AP 1:500) with K-562 cells lysate 1720 ug.

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  • KD/KO Validated

TRAPPC12 Polyclonal antibody

TRAPPC12 Polyclonal Antibody for WB, IP, ELISA
Cat No. 24555-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

TTC15, CGI 87, CGI-87, TPR repeat protein 15, Trafficking of membranes and mitosis

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inK-562 cells
Positive IP detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24555-1-AP targets TRAPPC12 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRAPPC12 fusion protein Ag18332 相同性解析による交差性が予測される生物種
Full Name tetratricopeptide repeat domain 15
Calculated molecular weight 735 aa, 79 kDa
Observed molecular weight98 kDa
GenBank accession numberBC014164
Gene Symbol TRAPPC12
Gene ID (NCBI) 51112
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WVT3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRAPPC12, also called CGI-87, TTC15 and TRAMM, has been recently identified as subunit of TRAPP complex in two independent proteomic studies. It was suggested that TRAPPC12 was a subunit of TRAPPIII. TRAPPC12 and TECPR1 acted at distinct steps in autophagy and depletion of TRAPPC12 and/or TECPR1 increased in the number of autophagosomes and increase autophagic flux. The observed molecular weight of TRAPPC12 is 98 kDa, which is consistent with what has been described in the literature (PMID: 28240221, PMID: 38439956).

Protocols

Product Specific Protocols
WB protocol for TRAPPC12 antibody 24555-1-APDownload protocol
IP protocol for TRAPPC12 antibody 24555-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols