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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRAPPC11 Polyclonal antibody (27774-1-AP)

    WB analysis using 27774-1-AP (same clone as 27774-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27774-1-AP (TRAPPC11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27774-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using TRAPPC11 Polyclonal antibody (27774-1-AP)

    IP experiment of HeLa using 27774-1-AP (same clone as 27774-1-PBS)

    IP result of anti-TRAPPC11 (IP:27774-1-AP, 4ug; Detection:27774-1-AP 1:500) with HeLa cells lysate 1320 ug. This data was developed using the same antibody clone with 27774-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using TRAPPC11 Polyclonal antibody (27774-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 27774-1-AP (same clone as 27774-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27774-1-AP (TRAPPC11 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27774-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using TRAPPC11 Polyclonal antibody (27774-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 27774-1-AP (same clone as 27774-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27774-1-AP (TRAPPC11 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27774-1-PBS in a different storage buffer formulation.

TRAPPC11 Polyclonal antibody, PBS Only

TRAPPC11 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 27774-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

C4orf41, UPF0636 protein C4orf41, Trafficking protein particle complex subunit 11

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27774-1-PBS targets TRAPPC11 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag27096

Product name: Recombinant human C4orf41 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 519-735 aa of BC022185

Sequence: LLGRASTLKDDQKSRIEKNLINVLMNESPDPEPDCDILAVKTAQKLWADRISLAGSNIFTIGVQDFVPFVQCKAKFHAPSFHVDVPVQFDIYLKADCPHPIRFSKLCVSFNNQEYNQFCVIEEASKANEVLENLTQGKMCLVPGKTRKLLFKFVAKTEDVGKKIEITSVDLALGNETGRCVVLNWQGGGGDAASSQEALQAARSFKRRPKLPDNEVH

相同性解析による交差性が予測される生物種
Full Name chromosome 4 open reading frame 41
Observed molecular weight129 kDa
GenBank accession numberBC022185
Gene Symbol TRAPPC11
Gene ID (NCBI) 60684
RRIDAB_3085995
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z392
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TRAPPC11 is a subunit of the TRAPP (transport protein particle) tethering complex, which functions in intracellular vesicle trafficking. This subunit is involved in early stage endoplasmic reticulum-to-Golgi vesicle transport. Mutations in TRAPPC11 result in neuromuscular and developmental phenotypes.

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