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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRAPPC1 Polyclonal antibody (19598-1-AP)

    WB analysis using 19598-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19598-1-AP (TRAPPC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TRAPPC1 Polyclonal antibody (19598-1-AP)

    IHC staining of mouse brain using 19598-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19598-1-AP (TRAPPC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TRAPPC1 Polyclonal antibody (19598-1-AP)

    IHC staining of mouse brain using 19598-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19598-1-AP (TRAPPC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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TRAPPC1 Polyclonal antibody

TRAPPC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 19598-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

BET5, BET5 homolog, Multiple myeloma protein 2, MUM2, MUM-2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, K-562 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19598-1-AP targets TRAPPC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRAPPC1 fusion protein Ag5608 相同性解析による交差性が予測される生物種
Full Name trafficking protein particle complex 1
Calculated molecular weight 145 aa, 17 kDa
Observed molecular weight 15-17 kDa
GenBank accession numberBC032717
Gene Symbol TRAPPC1
Gene ID (NCBI) 58485
RRIDAB_3085582
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y5R8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRAPPC1, also known as BET5 or MUM2, is a part of the multisubunit TRAPP (transport protein particle) complexes which are involved in the tethering process at different trafficking steps of vesicle transport (PMID: 16828797). TRAPPC1 may play a role in vesicular transport from the endoplasmic reticulum to Golgi (PMID: 10582700).

Protocols

Product Specific Protocols
WB protocol for TRAPPC1 antibody 19598-1-APDownload protocol
IHC protocol for TRAPPC1 antibody 19598-1-APDownload protocol
IF protocol for TRAPPC1 antibody 19598-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols