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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRAPA/SSR1 Monoclonal antibody (67925-1-Ig)

    WB analysis using 67925-1-Ig (same clone as 67925-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67925-1-Ig (TRAPA/SSR1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67925-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using TRAPA/SSR1 Monoclonal antibody (67925-1-Ig)

    IHC staining of human ovary tumor using 67925-1-Ig (same clone as 67925-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67925-1-Ig (TRAPA/SSR1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67925-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using TRAPA/SSR1 Monoclonal antibody (67925-1-Ig)

    IHC staining of human ovary tumor using 67925-1-Ig (same clone as 67925-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67925-1-Ig (TRAPA/SSR1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67925-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human ovary tumor tissue using TRAPA/SSR1 Monoclonal antibody (67925-1-Ig)

    IF Staining of human ovary tumor using 67925-1-Ig (same clone as 67925-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using TRAPA/SSR1 antibody (67925-1-Ig, Clone: 1F2D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67925-1-PBS in a different storage buffer formulation.

TRAPA/SSR1 Monoclonal antibody, PBS Only

TRAPA/SSR1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 67925-1-PBS
Clone No.1F2D7

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

DKFZp781N23103, FLJ14232, FLJ22100, FLJ23034, FLJ78242, FLJ93042, SSR alpha, SSR1, TRAP alpha, TRAPA

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67925-1-PBS targets TRAPA/SSR1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag30832

Product name: Recombinant human TRAPA, SSR1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-286 aa of BC007710

Sequence: MRLLPRLLLLLLLVFPATVLFRGGPRGLLAVAQDLTEDEETVEDSIIEDEDDEAEVEEDEPTDLVEDKEEEDVSGEPEASPSADTTILFVKGEDFPANNIVKFLVGFTNKGTEDFIVESLDASFRYPQDYQFYIQNFTALPLNTVVPPQRQATFEYSFIPAEPMGGRPFGLVINLNYKDLNGNVFQDAVFNQTVTVIEREDGLDGETIFMYMFLAGLGLLVIVGLHQLLESRKRKRPIQKVEMGTSSQNDVDMSWIPQETLNQINKASPRRLPRKRAQKRSVGSDE

相同性解析による交差性が予測される生物種
Full Name signal sequence receptor, alpha
Calculated molecular weight 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC007710
Gene Symbol TRAPA
Gene ID (NCBI) 6745
RRIDAB_2918677
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP43307
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TRAPA (translocon-associated protein subunit alpha), also known as SSR1 (signal sequence receptor subunit alpha), is a 34-kDa single-spanning membrane glycoprotein of the endoplasmic reticulum (ER) (PMID: 8050590). It is a part of the tetrameric TRAP complex (TRAP-alpha, TRAP-beta, TRAP-delta and TRAP-gamma) which functions in regulating the retention of ER resident proteins (PMID: 7916687). The TRAP complex is also involved in endoplasmic reticulum-associated degradation (ERAD) (PMID: 17380188).