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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRAM1 Polyclonal antibody

TRAM1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12705-1-AP

Synonyms

PNAS 8, PRO1292, TRAM, TRAM1, TRAMP

Validation Data Gallery

Applications:
  • WB analysis of K-562 using 12705-1-AP

    WB analysis of K-562 using 12705-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 12705-1-AP (TRAM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 12705-1-AP

    WB analysis of HeLa using 12705-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12705-1-AP (TRAM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 12705-1-AP

    WB analysis of Jurkat using 12705-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12705-1-AP (TRAM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of PC-3 using 12705-1-AP

    WB analysis of PC-3 using 12705-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 12705-1-AP (TRAM1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 12705-1-AP

    IHC staining of human prostate cancer using 12705-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12705-1-AP (TRAM1 antibody) at dilution of 1:50 (under 10x lens).

  • IF Staining of PC-3 using 12705-1-AP

    IF Staining of PC-3 using 12705-1-AP

    Immunofluorescent analysis of PC-3 cells using 12705-1-AP (TRAM1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inK-562 cells, HeLa cells, Jurkat cells, PC-3 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

12705-1-AP targets TRAM1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRAM1 fusion protein Ag3384 相同性解析による交差性が予測される生物種
Full Name translocation associated membrane protein 1
Calculated molecular weight 374 aa, 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC037738
Gene symbol TRAM1
Gene ID (NCBI) 23471
RRIDAB_2282651
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TRAM1 antibody 12705-1-APDownload protocol
IHC protocol for TRAM1 antibody 12705-1-APDownload protocol
IF protocol for TRAM1 antibody 12705-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Front Pharmacol

Achyranthes bidentata Polysaccharide Activates Nuclear Factor-Kappa B and Promotes Cytokine Production in J774A.1 Cells Through TLR4/MyD88 Signaling Pathway

Authors - Sairong Fan
View Article
ratWB

Evid Based Complement Alternat Med

The Herbal Medicine Scutellaria-Coptis Alleviates Intestinal Mucosal Barrier Damage in Diabetic Rats by Inhibiting Inflammation and Modulating the Gut Microbiota.

Authors - Boxun Zhang
View Article