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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TRAIL/CD253 Polyclonal antibody (27064-1-AP)

    WB analysis using 27064-1-AP (same clone as 27064-1-PBS)

    Human milk sample were subjected to SDS PAGE followed by western blot with 27064-1-AP (TRAIL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27064-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TRAIL/CD253 Polyclonal antibody (27064-1-AP)

    WB analysis using 27064-1-AP (same clone as 27064-1-PBS)

    Human milk were subjected to SDS PAGE followed by western blot with 27064-1-AP (TRAIL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27064-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using TRAIL/CD253 Polyclonal antibody (27064-1-AP)

    IHC staining of human kidney using 27064-1-AP (same clone as 27064-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27064-1-AP (TRAIL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27064-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using TRAIL/CD253 Polyclonal antibody (27064-1-AP)

    IHC staining of human kidney using 27064-1-AP (same clone as 27064-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27064-1-AP (TRAIL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27064-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using TRAIL/CD253 Polyclonal antibody (27064-1-AP)

    IHC staining of human lymphoma using 27064-1-AP (same clone as 27064-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27064-1-AP (TRAIL antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27064-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using TRAIL/CD253 Polyclonal antibody (27064-1-AP)

    IHC staining of human lymphoma using 27064-1-AP (same clone as 27064-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27064-1-AP (TRAIL antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27064-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using TRAIL/CD253 Polyclonal antibody (27064-1-AP)

    IF Staining of MCF-7 using 27064-1-AP (same clone as 27064-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 27064-1-AP (TRAIL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27064-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

TRAIL/CD253 Polyclonal antibody, PBS Only

TRAIL/CD253 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 27064-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

TNFSF10, TRAIL, Apo-2 ligand, CD253, Protein TRAIL

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27064-1-PBS targets TRAIL/CD253 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRAIL/CD253 fusion protein Ag25746 相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor (ligand) superfamily, member 10
Calculated molecular weight 281 aa, 33 kDa
Observed molecular weight 33 kDa, 20 kDa
GenBank accession numberBC032722
Gene Symbol TRAIL
Gene ID (NCBI) 8743
RRIDAB_2880737
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP50591
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TRAIL, also known as CD253 or TNFSF10 (tumor necrosis factor superfamily member 10), is a typical death ligand expressed on natural killer cells and cytotoxic T lymphocytes. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL induces apoptotic cell death in cancer by binding to its functional death receptors, death receptor (DR) 4 (TNFRSF10A/TRAIL-R1) and DR5 (TNFRSF10B/TRAIL-R2) to activate the extrinsic apoptosis pathway. TRAIL also activates c-Jun N-terminal kinase (MAPK8/JNK) and the transcription factor nuclear factor-κB (NFκB). The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3.