Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, human colon cancer tissue, mouse thymus tissue |
| Positive IHC detected in | human colon cancer tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 2 publications below |
Product Information
18110-1-AP targets TRAF3IP3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12559 Product name: Recombinant human TRAF3IP3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-353 aa of BC110302 Sequence: MISPDPRPSPGLARWAESYEAKCERRQEIRESRRCRPNVTTCRQVGKTLRIQQREQLQRARLQQFFRRRNLELEEKGKAQHPQAREQGPSRRPGQVTVLKEPLSCARRISSPREQVTGTSSEVFPAQHPPPSGICRDLSDHLSSQAGGLPPQDTPIKKPPKHHRGTQTKAEGPTIKNDASQQTNYGVAVLDKEIIQLSDYLKEALQRELVLKQKMVILQDLLSTLIQASDSSWKGQLNEDKLKGKLRSLENQLYTCTQKYSPWGMKKVLLEMEDQKNSYEQKAKESLQKVLEEKMNAEQQLQSTQRSLALAEQKCEEWRSQYEALKEDWRTLGTQHRELESQLHVLQSKLQIN 相同性解析による交差性が予測される生物種 |
| Full Name | TRAF3 interacting protein 3 |
| Calculated molecular weight | 353aa,41 kDa; 551aa,64 kDa |
| Observed molecular weight | 64 kDa, 47 kDa, 41 kDa |
| GenBank accession number | BC110302 |
| Gene Symbol | TRAF3IP3 |
| Gene ID (NCBI) | 80342 |
| RRID | AB_2918057 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y228 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TRAF3 interacting protein 3 (TRAF3IP3) is an essential adapter protein containing a coiled-coil domain that affects both innate and adaptive immunity. It plays a crucial role in the regulation of thymocyte development (PMID:26195727), and it also regulates T-cell stability and function by recruiting the serine-threonine phosphatase catalytic subunit (PPP2CA) to the lysosome, thereby facilitating the interaction of PP2Ac with the mTORC1 component RPTOR and restricting glycolytic metabolism (PMID:30115741). TRAF3IP3 responses to viral infection, facilitates the recruitment of TRAF3 to MAVS within mitochondria leading to IRF3 activation and interferon production (PMID:31390091). TRAF3IP3 has 3 isoforms produced by alternative splicing with the molecular weight of 64 kDa, 47 kDa and 41 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TRAF3IP3 antibody 18110-1-AP | Download protocol |
| WB protocol for TRAF3IP3 antibody 18110-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Braz J Med Biol Res SNORA5A regulates tumor-associated macrophage M1/M2 phenotypes via TRAF3IP3 in breast cancer | ||
Adv Sci (Weinh) TRAF3IP3 Induces ER Stress-Mediated Apoptosis with Protective Autophagy to Inhibit Lung Adenocarcinoma Proliferation |
