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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRAF1 Polyclonal antibody

TRAF1 Polyclonal Antibody for IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26845-1-AP

Synonyms

EBI6, TRAF1

Validation Data Gallery

Applications:
  • IHC staining of human tonsillitis using 26845-1-AP

    IHC staining of human tonsillitis using 26845-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26845-1-AP (TRAF1 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human tonsillitis using 26845-1-AP

    IHC staining of human tonsillitis using 26845-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26845-1-AP (TRAF1 Antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human spleen using 26845-1-AP

    IHC staining of human spleen using 26845-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 26845-1-AP (TRAF1 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human spleen using 26845-1-AP

    IHC staining of human spleen using 26845-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 26845-1-AP (TRAF1 Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HUVEC using 26845-1-AP

    IF Staining of HUVEC using 26845-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HUVEC cells using 26845-1-AP (TRAF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of Raji using 26845-1-AP

    FC experiment of Raji using 26845-1-AP

    1X10^6 Raji cells were intracellularly stained with 0.20 ug/test Anti-Human TRAF1 (26845-1-AP) (red) or 0.20 ug control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000. Fixed with 90% MeOH.

  • FC experiment of Raji using 26845-1-AP

    FC experiment of Raji using 26845-1-AP

    1X10^6 Raji cells were surface stained with 0.5 ug Anti-Human TRAF1 (26845-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.5 ug Control Antibody. Cells were fixed with 90% MeOH.

Tested Applications

Positive IHC detected inhuman tonsillitis tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHUVEC cells
Positive FC detected inRaji cells
Planning an IF experiment? We recommend our CoraLite® Plus 647 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 26845-1-AP is TRAF1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRAF1 fusion protein Ag25397 相同性解析による交差性が予測される生物種
Full Name TNF receptor-associated factor 1
Calculated molecular weight 416 aa, 46 kDa
GenBank accession numberBC024145
Gene symbol TRAF1
Gene ID (NCBI) 7185
RRIDAB_2880655
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for TRAF1 antibody 26845-1-APDownload protocol
IF protocol for TRAF1 antibody 26845-1-APDownload protocol
FC protocol for TRAF1 antibody 26845-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Lett

Up-regulation of OLR1 expression by TBC1D3 through activation of TNFα/NF-κB pathway promotes the migration of human breast cancer cells.

Authors - Bei Wang
View Article
ratWB

J Cell Mol Med

Exercise ameliorates insulin resistance and improves ASK1-mediated insulin signalling in obese rats.

Authors - Yong Zhang
View Article
humanWB

Front Pharmacol

Anthraquinone Emodin Inhibits Tumor Necrosis Factor Alpha-Induced Calcification of Human Aortic Valve Interstitial Cells via the NF-κB Pathway.

Authors - Kang Xu
View Article
humanWB

Int Immunopharmacol

Autoinducer-2 of Fusobacterium nucleatum promotes macrophage M1 polarization via TNFSF9/IL-1β signaling.

Authors - Jiao Wu
View Article
humanWB

Int Urol Nephrol

TRAIP suppressed apoptosis and cell cycle to promote prostate cancer proliferation via TRAF2-PI3K-AKT pathway activation

Authors - Yuan Gu
View Article
humanWB,IHC

J Exp Clin Cancer Res

DNMT1-induced miR-378a-3p silencing promotes angiogenesis via the NF-κB signaling pathway by targeting TRAF1 in hepatocellular carcinoma

Authors - Bin Zhu
View Article