Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, human testis tissue, mouse brain tissue, rat brain tissue |
| Positive IP detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33633-1-AP targets TRABD in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag40480 Product name: Recombinant human TRABD protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-85 aa of BC093029 Sequence: MDGEEQQPPHEANVEPVVPSEASEPVPRVLSGDPQNLSDVDAFNLLLEMKLKRRRQRPNLPRTVTQLVAEDGSRVYVVGTAHFSD 相同性解析による交差性が予測される生物種 |
| Full Name | TraB domain containing |
| Observed molecular weight | 37 kDa |
| GenBank accession number | BC093029 |
| Gene Symbol | TRABD |
| Gene ID (NCBI) | 80305 |
| RRID | AB_3743034 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9H4I3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TraB domain-containing protein (TRABD) is also named TTG2. TRABD is a Tiki/TraB family protein that is highly expressed in the brains of patients with AD (PMID: 33446646). TRABD as a novel outer mitochondrial membrane protein. Depletion of TRABD in cells severely impairs mitochondrial respiration and ATP production, inhibits cell growth, increases reactive oxygen species levels (PMID: 40778267).
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for TRABD antibody 33633-1-AP | Download protocol |
| WB protocol for TRABD antibody 33633-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |