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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TPX2 Monoclonal antibody (68712-1-Ig)

    WB analysis using 68712-1-Ig (same clone as 68712-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68712-1-Ig (TPX2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68712-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TPX2 Monoclonal antibody (68712-1-Ig)

    WB analysis using 68712-1-Ig (same clone as 68712-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68712-1-Ig (TPX2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68712-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using TPX2 Monoclonal antibody (68712-1-Ig)

    WB analysis of HEK-293 using 68712-1-Ig (same clone as 68712-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 68712-1-Ig (TPX2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68712-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using TPX2 Monoclonal antibody (68712-1-Ig)

    WB analysis of Jurkat using 68712-1-Ig (same clone as 68712-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 68712-1-Ig (TPX2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68712-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using TPX2 Monoclonal antibody (68712-1-Ig)

    IF Staining of A549 using 68712-1-Ig (same clone as 68712-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using TPX2 antibody (68712-1-Ig, Clone: 2E10C1 ) at dilution of 1:4000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68712-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using TPX2 Monoclonal antibody (68712-1-Ig)

    IF Staining of U2OS using 68712-1-Ig (same clone as 68712-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using TPX2 antibody (68712-1-Ig, Clone: 2E10C1 ) at dilution of 1:4000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68712-1-PBS in a different storage buffer formulation.

TPX2 Monoclonal antibody, PBS Only

TPX2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68712-1-PBS
Clone No.2E10C1

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

2E10C1, C20orf1, C20orf2, Differentially expressed in cancerous and non-cancerous lung cells 2, DIL 2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68712-1-PBS targets TPX2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag33683

Product name: Recombinant human TPX2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-395 aa of BC020207

Sequence: MSQVKSSYSYDAPSDFINFSSLDDEGDTQNIDSWFEEKANLENKLLGKNGTGGLFQGKTPLRKANLQQAIVTPLKPVDNTYYKEAEKENLVEQSIPSNACSSLEVEAAISRKTPAQPQRRSLRLSAQKDLEQKEKHHVKMKAKRCATPVIIDEILPSKKMKVSNNKKKPEEEGSAHQDTAEKNASSPEKAKGRHTVPCMPPAKQKFLKSTEEQELEKSMKMQQEVVEMRKKNEEFKKLALAGIGQPVKKSVSQVTKSVDFHFRTDERIKQHPKNQEEYKEVNFTSELRKHPSSPARVTKGCTIVKPFNLSQGKKRTFDETVSTYVPLAQQVEDFHKRTPNRYHLRSKKDDINLLPSKSSVTKICRDPQTPVLQTKHRARAVTCKSTAELEAEELE

相同性解析による交差性が予測される生物種
Full Name TPX2, microtubule-associated, homolog (Xenopus laevis)
Calculated molecular weight 747 aa, 86 kDa
Observed molecular weight 98 kDa
GenBank accession numberBC020207
Gene Symbol TPX2
Gene ID (NCBI) 22974
RRIDAB_3670405
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9ULW0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TPX2, also known as DIL2, p10, is a microtubule organization- and cell cycle-associated protein. In the early stage of mitosis, TPX2 is released in a RanGTP-dependent manner and plays a significant role in mitotic spindle formation and subsequent proper segregation of chromosomes during cell division. Furthermore, during interphase, TPX2 is involved in DNA damage response by regulation of γ-H2AX signals(PMID: 25914189). Aberrant expression of TPX2 leads to improper spindle assembly and chromosomal instability. Overexpressed in cancers, TPX2 is being established as the marker for the diagnosis and prognosis of malignancies(PMID: 24556998).

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