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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TPX2 Polyclonal antibody (11741-1-AP)

    WB analysis using 11741-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11741-1-AP (TPX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using TPX2 Polyclonal antibody (11741-1-AP)

    WB analysis of HepG2 using 11741-1-AP

    WB result of TPX2 antibody (11741-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPX2 transfected HepG2 cells.

  • Western Blot (WB) analysis of K-562 cells using TPX2 Polyclonal antibody (11741-1-AP)

    WB analysis of K-562 using 11741-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 11741-1-AP (TPX2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using TPX2 Polyclonal antibody (11741-1-AP)

    IP experiment of HeLa using 11741-1-AP

    IP result of anti-TPX2 (IP:11741-1-AP, 4ug; Detection:11741-1-AP 1:4000) with HeLa cells lysate 1760 ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TPX2 Polyclonal antibody (11741-1-AP)

    IHC staining of human colon cancer using 11741-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11741-1-AP (TPX2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TPX2 Polyclonal antibody (11741-1-AP)

    IHC staining of human colon cancer using 11741-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11741-1-AP (TPX2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using TPX2 Polyclonal antibody (11741-1-AP)

    IHC staining of human prostate cancer using 11741-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11741-1-AP (TPX2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TPX2 Polyclonal antibody (11741-1-AP)

    IHC staining of human breast cancer using 11741-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11741-1-AP (TPX2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TPX2 Polyclonal antibody (11741-1-AP)

    IHC staining of human breast cancer using 11741-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11741-1-AP (TPX2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TPX2 Polyclonal antibody (11741-1-AP)

    IF Staining of HeLa using 11741-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11741-1-AP (TPX2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TPX2 Polyclonal antibody (11741-1-AP)

    IF Staining of HepG2 using 11741-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TPX2 antibody (11741-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TPX2 Polyclonal antibody (11741-1-AP)

    IF Staining of HeLa using 11741-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11741-1-AP (TPX2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO Validated

TPX2 Polyclonal antibody

TPX2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11741-1-AP
Publications(33)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Synonyms

C20orf1, C20orf2, Differentially expressed in cancerous and non-cancerous lung cells 2, DIL 2, DIL2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, K-562 cells, HeLa cells, SMMC-7721 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11741-1-AP targets TPX2 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2334

Product name: Recombinant human TPX2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-395 aa of BC020207

Sequence: MSQVKSSYSYDAPSDFINFSSLDDEGDTQNIDSWFEEKANLENKLLGKNGTGGLFQGKTPLRKANLQQAIVTPLKPVDNTYYKEAEKENLVEQSIPSNACSSLEVEAAISRKTPAQPQRRSLRLSAQKDLEQKEKHHVKMKAKRCATPVIIDEILPSKKMKVSNNKKKPEEEGSAHQDTAEKNASSPEKAKGRHTVPCMPPAKQKFLKSTEEQELEKSMKMQQEVVEMRKKNEEFKKLALAGIGQPVKKSVSQVTKSVDFHFRTDERIKQHPKNQEEYKEVNFTSELRKHPSSPARVTKGCTIVKPFNLSQGKKRTFDETVSTYVPLAQQVEDFHKRTPNRYHLRSKKDDINLLPSKSSVTKICRDPQTPVLQTKHRARAVTCKSTAELEAEELE

相同性解析による交差性が予測される生物種
Full Name TPX2, microtubule-associated, homolog (Xenopus laevis)
Calculated molecular weight 747 aa, 86 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC020207
Gene Symbol TPX2
Gene ID (NCBI) 22974
RRIDAB_2208895
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9ULW0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TPX2, also known as DIL2, p10, is a microtubule organization- and cell cycle-associated protein. In the early stage of mitosis, TPX2 is released in a RanGTP-dependent manner and plays a significant role in mitotic spindle formation and subsequent proper segregation of chromosomes during cell division. Furthermore, during interphase, TPX2 is involved in DNA damage response by regulation of γ-H2AX signals(PMID: 25914189). Aberrant expression of TPX2 leads to improper spindle assembly and chromosomal instability. Overexpressed in cancers, TPX2 is being established as the marker for the diagnosis and prognosis of malignancies(PMID: 24556998).

Protocols

Product Specific Protocols
IF protocol for TPX2 antibody 11741-1-APDownload protocol
IHC protocol for TPX2 antibody 11741-1-APDownload protocol
IP protocol for TPX2 antibody 11741-1-APDownload protocol
WB protocol for TPX2 antibody 11741-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Mol Cell

The RNA-stability-independent role of the RNA m6A reader YTHDF2 in promoting protein translation to confer tumor chemotherapy resistance

Authors - Tao Liu
View Article
mouseWB

Adv Sci (Weinh)

Arf1 GTPase Regulates Golgi-Dependent G2/M Transition and Spindle Organization in Oocyte Meiosis

Authors - Kun-Huan Zhang
View Article
human, mouseIHC,IF

Cell Rep Med

Targeting TPX2-dependent lineage plasticity by CDK4/6 inhibition reverses therapy resistance in neuroendocrine bladder carcinoma.

Authors - Zehua Chen
  • KD Validated
View Article
mouseIF

Cell Commun Signal

Tubacin alleviate the reproductive toxicity of deoxynivalenol in mouse oocytes and zygotes via strengthening microtubule stability.

Authors - Hui Luo
View Article

Sci China Life Sci

Insufficient MIRO1 contributes to declined oocyte quality during reproductive aging

Authors - Zhen-Nan Pan
View Article
humanWB

Cell Chem Biol

Non-canonical function of histone methyltransferase G9a in the translational regulation of chronic inflammation

Authors - Adil Muneer
View Article
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