Validation Data Gallery
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Tested Applications
| Positive WB detected in | L02 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25120-1-AP targets TPTE2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19169 Product name: Recombinant human TPTE2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-62 aa of BC128146 Sequence: MNESPQTNEFKGTTEEAPAKESPHTSEFKGAALVSPISKSMLERLSKFEVEDAENVASYEWT 相同性解析による交差性が予測される生物種 |
| Full Name | transmembrane phosphoinositide 3-phosphatase and tensin homolog 2 |
| Calculated molecular weight | 522 aa, 61 kDa |
| Observed molecular weight | 38-60 kDa |
| GenBank accession number | BC128146 |
| Gene Symbol | TPTE2 |
| Gene ID (NCBI) | 93492 |
| RRID | AB_2879908 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6XPS3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TPTE2 antibody 25120-1-AP | Download protocol |
| IHC protocol for TPTE2 antibody 25120-1-AP | Download protocol |
| WB protocol for TPTE2 antibody 25120-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |