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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TPST2 Polyclonal antibody (30426-1-AP)

    WB analysis using 30426-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30426-1-AP (TPST2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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TPST2 Polyclonal antibody

TPST2 Polyclonal Antibody for WB, ELISA
Cat No. 30426-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

TPST-2, Protein-tyrosine sulfotransferase 2, EC:2.8.2.20

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30426-1-AP targets TPST2 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TPST2 fusion protein Ag32362 相同性解析による交差性が予測される生物種
Full Name tyrosylprotein sulfotransferase 2
Calculated molecular weight 377 aa, 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC017509
Gene Symbol TPST2
Gene ID (NCBI) 8459
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60704
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TPST2 catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides, using 3'-phosphoadenylyl sulfate (PAPS) as cosubstrate (PMID: 9733778).

Protocols

Product Specific Protocols
WB protocol for TPST2 antibody 30426-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols