Validation Data Gallery
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Tested Applications
| Positive WB detected in | A431 cells, K-562 cells, mouse liver tissue, rat liver tissue |
| Positive IP detected in | mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IHC | See 5 publications below |
| IF | See 7 publications below |
Product Information
13343-1-AP targets TPSAB1/TPSB2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4167 Product name: Recombinant human TPSAB1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-275 aa of BC028059 Sequence: MLSLLLLALPVLASPAYAAPAPVQALQQAGIVGGQEAPRSKWPWQVSLRVRDRYWMHFCGGSLIHPQWVLTAAHCLGPDVKDLATLRVQLREQHLYYQDQLLPVSRIIVHPQFYIIQTGADIALLELEEPVNISSRVHTVMLPPASETFPPGMPCWVTGWGDVDNDEPLPPPFPLKQVKVPIMENHICDAKYHLGAYTGDDVRIIRDDMLCAGNTRRDSCQGDSGGPLVCKVNGTWLQAGVVSWDEGCAQPNRPGIYTRVTYYLDWIHHYVPKKP 相同性解析による交差性が予測される生物種 |
| Full Name | tryptase alpha/beta 1 |
| Calculated molecular weight | 275 aa, 31 kDa |
| Observed molecular weight | 32-38 kDa |
| GenBank accession number | BC028059 |
| Gene Symbol | TPSAB1 |
| Gene ID (NCBI) | 7177 |
| RRID | AB_2206197 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15661 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for TPSAB1/TPSB2 antibody 13343-1-AP | Download protocol |
| WB protocol for TPSAB1/TPSB2 antibody 13343-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Clin Transl Med Integrating single-cell RNA sequencing with spatial transcriptomics reveals an immune landscape of human myometrium during labour | ||
Front Immunol Palmatine treats urticaria by reducing inflammation and increasing autophagy | ||
Int Immunopharmacol Cold-inducible RNA-binding protein promotes gut barrier dysfunction and visceral hypersensitivity via activating mast cells in IBS-D | ||
IUBMB Life Bromodomain-containing protein 9 is a prognostic biomarker associated with immune infiltrates and promotes tumor malignancy through activating notch signaling pathway in negative HIF-2α clear cell renal cell carcinoma. | ||
BMC Cancer The release of tryptase from mast cells promote tumor cell metastasis via exosomes. | ||
Shock Activation of intestinal mast cells contributes to gut damage after cardiac arrest in mice |