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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TPPP Monoclonal antibody (68048-1-Ig)

    WB analysis using 68048-1-Ig (same clone as 68048-1-PBS)

    Brain tissues were subjected to SDS PAGE followed by western blot with 68048-1-Ig (TPPP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68048-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using TPPP Monoclonal antibody (68048-1-Ig)

    WB analysis of SH-SY5Y using 68048-1-Ig (same clone as 68048-1-PBS)

    WB result of TPPP antibody (68048-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPPP transfected SH-SY5Y cells. This data was developed using the same antibody clone with 68048-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig cerebellum tissue using TPPP Monoclonal antibody (68048-1-Ig)

    WB analysis of pig cerebellum using 68048-1-Ig (same clone as 68048-1-PBS)

    pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 68048-1-Ig (TPPP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68048-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rabbit cerebellum tissue using TPPP Monoclonal antibody (68048-1-Ig)

    WB analysis of rabbit cerebellum using 68048-1-Ig (same clone as 68048-1-PBS)

    rabbit cerebellum tissue were subjected to SDS PAGE followed by western blot with 68048-1-Ig (TPPP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68048-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat cerebellum tissue using TPPP Monoclonal antibody (68048-1-Ig)

    WB analysis of rat cerebellum using 68048-1-Ig (same clone as 68048-1-PBS)

    rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 68048-1-Ig (TPPP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68048-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using TPPP Monoclonal antibody (68048-1-Ig)

    IHC staining of human lung using 68048-1-Ig (same clone as 68048-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 68048-1-Ig (TPPP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68048-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using TPPP Monoclonal antibody (68048-1-Ig)

    IHC staining of human lung using 68048-1-Ig (same clone as 68048-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 68048-1-Ig (TPPP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68048-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TPPP Monoclonal antibody (68048-1-Ig)

    IHC staining of mouse brain using 68048-1-Ig (same clone as 68048-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68048-1-Ig (TPPP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68048-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TPPP Monoclonal antibody (68048-1-Ig)

    IHC staining of mouse brain using 68048-1-Ig (same clone as 68048-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68048-1-Ig (TPPP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68048-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Neuro-2a cells using TPPP Monoclonal antibody (68048-1-Ig)

    IF Staining of Neuro-2a using 68048-1-Ig (same clone as 68048-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using TPPP antibody (68048-1-Ig, Clone: 1E5F5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68048-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

TPPP Monoclonal antibody, PBS Only

TPPP Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 68048-1-PBS
Clone No.1E5F5

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit, chicken

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

p25 alpha, p24, EC:3.6.5.-, 25 kDa brain-specific protein, 25 kDa brain specific protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68048-1-PBS targets TPPP in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit, chicken samples.

Tested Reactivity human, mouse, rat, pig, rabbit, chicken
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TPPP fusion protein Ag19046 相同性解析による交差性が予測される生物種
Full Name tubulin polymerization promoting protein
Calculated molecular weight 219 aa, 24 kDa
Observed molecular weight24-29 kDa
GenBank accession numberBC131506
Gene Symbol TPPP
Gene ID (NCBI) 11076
RRIDAB_2918790
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO94811
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

TPPP, also named as TPPP1, P24 and P25, belongs to the TPPP family. TPPP promotes in vitro the polymerization of tubulin into double-walled tubules and polymorphic aggregates or bundled stabilized microtubules blocks. When overexpressed, TPPP inhibits mitotic spindle assembly and nuclear envelope breakdown, apparently without affecting other cellular events.