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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using TPPII Monoclonal antibody (66017-1-Ig)

    WB analysis of Jurkat using 66017-1-Ig (same clone as 66017-1-PBS)

    WB result of TPPII antibody (66017-1-Ig, 1:4000) with si-control and si-TPPII transfected Jurkat cells. This data was developed using the same antibody clone with 66017-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using TPPII Monoclonal antibody (66017-1-Ig)

    WB analysis of HeLa using 66017-1-Ig (same clone as 66017-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66017-1-Ig (TPPII antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66017-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using TPPII Monoclonal antibody (66017-1-Ig)

    WB analysis of HepG2 using 66017-1-Ig (same clone as 66017-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66017-1-Ig (TPPII antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66017-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using TPPII Monoclonal antibody (66017-1-Ig)

    WB analysis of Raji using 66017-1-Ig (same clone as 66017-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 66017-1-Ig (TPPII antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66017-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using TPPII Monoclonal antibody (66017-1-Ig)

    IHC staining of human testis using 66017-1-Ig (same clone as 66017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 66017-1-Ig(TPPII antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66017-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using TPPII Monoclonal antibody (66017-1-Ig)

    IHC staining of human testis using 66017-1-Ig (same clone as 66017-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 66017-1-Ig(TPPII antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66017-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TPPII Monoclonal antibody (66017-1-Ig)

    IF Staining of HeLa using 66017-1-Ig (same clone as 66017-1-PBS)

    Immunofluorescent analysis of Hela cells, using TPP2 antibody 66017-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 66017-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using TPPII Monoclonal antibody (66017-1-Ig)

    IF Staining of MCF-7 using 66017-1-Ig (same clone as 66017-1-PBS)

    Immunofluorescent analysis of MCF-7 cells, using TPP2 antibody 66017-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 66017-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TPPII Monoclonal antibody, PBS Only

TPPII Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66017-1-PBS
Clone No.5H6F11

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

TPP 2, TPP2, TPPII, Tripeptidyl aminopeptidase, Tripeptidyl peptidase 2, tripeptidyl peptidase II

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66017-1-PBS targets TPPII in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag5296

Product name: Recombinant human TPP2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 900-1249 aa of BC039905

Sequence: LERLKDLPFIVSHRLSNTLSLDIHENHSFALLGKKKSSNLTLPPKYNQPFFVTSLPDDKIPKGAGPGCYLAGSLTLSKTELGKKADVIPVHYYLIPPPTKTKNGSKDKEKDSEKEKDLKEEFTEALRDLKIQWMTKLDSSDIYNELKETYPNYLPLYVARLHQLDAEKERMKRLNEIVDAANAVISHIDQTALAVYIAMKTDPRPDAATIKNDMDKQKSTLVDALCRKGCALADHLLHTQAQDGAISTDAEGKEEEGESPLDSLAETFWETTKWTDLFDNKVLTFAYKHALVNKMYGRGLKFATKLVEEKPTKENWKNCIQLMKLLGWTHCASFTENWLPIMYPPDYCVF

相同性解析による交差性が予測される生物種
Full Name tripeptidyl peptidase II
Calculated molecular weight 1249 aa, 138 kDa
Observed molecular weight 135 kDa
GenBank accession numberBC039905
Gene Symbol TPPII
Gene ID (NCBI) 7174
RRIDAB_11044195
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP29144
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TPPII(Tripeptidyl-peptidase 2) is an evolutionary conserved serine peptidase of the subtilisin family that removes tripeptides from the free NH2 terminus of oligopeptides. The 138-kDa TPP2 polypeptide forms large oligomeric complexes of >1,000 kDa that are detected in the cytoplasm of all cell types. In addition, a membrane associated TPP 2 isoform is present in most cells(PMID:15735022).

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