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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TPI1 Recombinant monoclonal antibody (88045-1-RR)

    WB analysis using 88045-1-RR (same clone as 88045-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 88045-1-RR (TPI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88045-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TPI1 Recombinant monoclonal antibody (88045-1-RR)

    WB analysis using 88045-1-RR (same clone as 88045-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 88045-1-RR (TPI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88045-1-PBS in a different storage buffer formulation.

TPI1 Recombinant monoclonal antibody, PBS Only

TPI1 Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 88045-1-PBS
Clone No.260189G12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

EC:4.2.3.3, EC:5.3.1.1, Methylglyoxal synthase, TIM, TPI

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

88045-1-PBS targets TPI1 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag1175

Product name: Recombinant human TPI1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-249 aa of BC007086

Sequence: MAPSRKFFVGGNWKMNGRKQSLGELIGTLNAAKVPADTEVVCAPPTAYIDFARQKLDPKIAVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVLGHSERRHVFGESDELIGQKVAHALAEGLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKSNVSDAVAQSTRIIYGGSVTGATCKELASQPDVDGFLVGGASLKPEFVDIINAKQ

相同性解析による交差性が予測される生物種
Full Name triosephosphate isomerase 1
Calculated molecular weight 27 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC007086
Gene Symbol TPI1
Gene ID (NCBI) 7167
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP60174
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TPI1(Triosephosphate isomerase) is also named as TIM, TPI and belongs to the triosephosphate isomerase family. It is a key enzyme in cell metabolism that controls the glycolytic flow and energy production through the interconversion of dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde 3-phosphate (G3P)(PMID:19251756). Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). It can exsit as a homodimer(PMID:18562316).

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