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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TPI1 Monoclonal antibody (67515-1-Ig)

    WB analysis using 67515-1-Ig (same clone as 67515-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67515-1-Ig (TPI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67515-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using TPI1 Monoclonal antibody (67515-1-Ig)

    WB analysis of HEK-293 using 67515-1-Ig (same clone as 67515-1-PBS)

    WB result of TPI1 antibody (67515-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPI1 transfected HEK-293 cells. This data was developed using the same antibody clone with 67515-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TPI1 Monoclonal antibody (67515-1-Ig)

    IHC staining of human colon cancer using 67515-1-Ig (same clone as 67515-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67515-1-Ig (TPI1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67515-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TPI1 Monoclonal antibody (67515-1-Ig)

    IHC staining of human colon cancer using 67515-1-Ig (same clone as 67515-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67515-1-Ig (TPI1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67515-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TPI1 Monoclonal antibody (67515-1-Ig)

    IF Staining of HeLa using 67515-1-Ig (same clone as 67515-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TPI1 antibody (67515-1-Ig, Clone: 2E7E5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67515-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TPI1 Monoclonal antibody, PBS Only

TPI1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67515-1-PBS
Clone No.2E7E5

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

TIM, TPI, TPI1, Triose phosphate isomerase, Triosephosphate isomerase, triosephosphate isomerase 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67515-1-PBS targets TPI1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag16653

Product name: Recombinant human TPI1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-249 aa of BC007086

Sequence: MAPSRKFFVGGNWKMNGRKQSLGELIGTLNAAKVPADTEVVCAPPTAYIDFARQKLDPKIAVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVLGHSERRHVFGESDELIGQKVAHALAEGLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKSNVSDAVAQSTRIIYGGSVTGATCKELASQPDVDGFLVGGASLKPEFVDIINAKQ

相同性解析による交差性が予測される生物種
Full Name triosephosphate isomerase 1
Calculated molecular weight 27 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC007086
Gene Symbol TPI1
Gene ID (NCBI) 7167
RRIDAB_2882736
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP60174
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TPI1(Triosephosphate isomerase) is also named as TIM, TPI and belongs to the triosephosphate isomerase family. It is a key enzyme in cell metabolism that controls the glycolytic flow and energy production through the interconversion of dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde 3-phosphate (G3P)(PMID:19251756). Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). It can exsit as a homodimer(PMID:18562316).