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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using TPI1 Monoclonal antibody (60036-2-Ig)

    WB analysis of HEK-293 using 60036-2-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 60036-2-Ig (TPI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TPI1 Monoclonal antibody (60036-2-Ig)

    WB analysis of HeLa using 60036-2-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 60036-2-Ig (TPI1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TPI1 Monoclonal antibody (60036-2-Ig)

    WB analysis of HeLa using 60036-2-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 60036-2-Ig (TPI1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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TPI1 Monoclonal antibody

TPI1 Monoclonal Antibody for WB, ELISA
Cat No. 60036-2-Ig
Clone No.3G11G3

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Synonyms

TIM, TPI, TPI1, Triose phosphate isomerase, Triosephosphate isomerase, triosephosphate isomerase 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60036-2-Ig targets TPI1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TPI1 fusion protein Ag1175 相同性解析による交差性が予測される生物種
Full Name triosephosphate isomerase 1
Calculated molecular weight 27 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC007086
Gene Symbol TPI1
Gene ID (NCBI) 7167
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP60174
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TPI1(Triosephosphate isomerase) is also named as TIM, TPI and belongs to the triosephosphate isomerase family. It is a key enzyme in cell metabolism that controls the glycolytic flow and energy production through the interconversion of dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde 3-phosphate (G3P)(PMID:19251756). Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). It can exsit as a homodimer(PMID:18562316).