• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using TPH2 Polyclonal antibody (29283-1-AP)

    WB analysis using 29283-1-AP

    Rat brain tissue were subjected to SDS PAGE followed by western blot with 29283-1-AP (TPH2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TPH2 Polyclonal antibody (29283-1-AP)

    WB analysis using 29283-1-AP

    Mouse brain tissue were subjected to SDS PAGE followed by western blot with 29283-1-AP (TPH2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TPH2 Polyclonal antibody (29283-1-AP)

    IHC staining of mouse brain using 29283-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29283-1-AP (TPH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using TPH2 Polyclonal antibody (29283-1-AP)

    IHC staining of rat brain using 29283-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 29283-1-AP (TPH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using TPH2 Polyclonal antibody (29283-1-AP)

    IHC staining of rat brain using 29283-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 29283-1-AP (TPH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TPH2 Polyclonal antibody (29283-1-AP)

    IHC staining of mouse brain using 29283-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29283-1-AP (TPH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

TPH2 Polyclonal antibody

TPH2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29283-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

EC:1.14.16.4, NTPH, TPH 2, Tryptophan 5-hydroxylase 2, Tryptophan 5-monooxygenase 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inrat brain tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29283-1-AP targets TPH2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TPH2 fusion protein Ag30281 相同性解析による交差性が予測される生物種
Full Name tryptophan hydroxylase 2
Calculated molecular weight 490 aa, 56 kDa
Observed molecular weight55 kDa
GenBank accession numberBC114442
Gene Symbol TPH2
Gene ID (NCBI) 121278
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IWU9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TPH2 protein is a key enzyme in the synthesis of serotonin in the brain. It is mainly expressed in the brain and is responsible for converting tryptophan to 5-hydroxytryptophan, a precursor of serotonin. TPH2 plays a crucial role in regulating mood, anxiety, and stress responses. Variations in the TPH2 gene have been associated with several psychiatric disorders, including depression and bipolar disorder. Additionally, TPH2 expression can be regulated by various factors, such as hormones and environmental stimuli (PMID: 22241550, PMID: 29775696, PMID: 32119710, PMID: 40204433).

Protocols

Product Specific Protocols
WB protocol for TPH2 antibody 29283-1-APDownload protocol
IHC protocol for TPH2 antibody 29283-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols