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  • Featured Product
  • KD/KO Validated

TPCN1 Polyclonal antibody

TPCN1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23758-1-AP

Synonyms

KIAA1169, TPC1, TPCN1, two pore segment channel 1

Validation Data Gallery

Applications:
  • WB analysis of mouse heart using 23758-1-AP

    WB analysis of mouse heart using 23758-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 23758-1-AP (TPCN1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 23758-1-AP

    IHC staining of human kidney using 23758-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23758-1-AP (TPCN1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 23758-1-AP

    IHC staining of human kidney using 23758-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23758-1-AP (TPCN1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 23758-1-AP

    IF Staining of HepG2 using 23758-1-AP

    Immunofluorescent analysis of HepG2 cells using 23758-1-AP (TPCN1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse heart tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 23758-1-AP is TPCN1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TPCN1 fusion protein Ag20607 相同性解析による交差性が予測される生物種
Full Name two pore segment channel 1
Calculated molecular weight 816 aa, 94 kDa
Observed molecular weight 94 kDa
GenBank accession numberBC136795
Gene symbol TPCN1
Gene ID (NCBI) 53373
RRIDAB_2879317
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TPCN1 antibody 23758-1-APDownload protocol
IHC protocol for TPCN1 antibody 23758-1-APDownload protocol
IF protocol for TPCN1 antibody 23758-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Sci Adv

CLN7 is an organellar chloride channel regulating lysosomal function.

Authors - Yayu Wang
  • KD Validated
View Article