Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HeLa using 14828-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14828-1-AP (PIG3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 14828-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 14828-1-AP (PIG3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of SMMC-7721 using 14828-1-AP
SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 14828-1-AP (PIG3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 14828-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 14828-1-AP (PIG3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14828-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14828-1-AP (PIG3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of MCF-7 using 14828-1-AP
IP result of anti-PIG3 (IP:14828-1-AP, 4ug; Detection:14828-1-AP 1:500) with MCF-7 cells lysate 3200ug.
IHC staining of human liver cancer using 14828-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14828-1-AP (PIG3 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 14828-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14828-1-AP (PIG3 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 14828-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PIG3 antibody (14828-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

PIG3 Polyclonal antibody

PIG3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 14828-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

TP53I3, Quinone oxidoreductase PIG3, p53-induced gene 3 protein, p53 induced gene 3 protein, NADPH:quinone reductase PIG3

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, HepG2 cells, MCF-7 cells, SMMC-7721 cells
Positive IP detected in	MCF-7 cells
Positive IHC detected in	human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IF	See 1 publications below

Product Information

14828-1-AP targets PIG3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PIG3 fusion protein Ag6618 相同性解析による交差性が予測される生物種
Full Name	tumor protein p53 inducible protein 3
Calculated molecular weight	35 kDa
Observed molecular weight	34-36 kDa
GenBank accession number	BC000474
Gene Symbol	TP53I3
Gene ID (NCBI)	9540
RRID	AB_2206893
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q53FA7
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for PIG3 antibody 14828-1-AP	Download protocol
IHC protocol for PIG3 antibody 14828-1-AP	Download protocol
IF protocol for PIG3 antibody 14828-1-AP	Download protocol
IP protocol for PIG3 antibody 14828-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF	Biomaterials Furin-instructed aggregated gold nanoparticles for re-educating tumor associated macrophages and overcoming breast cancer chemoresistance. Authors - Rou Xie View Article
human	WB	Virology VP5 protein of oncolytic herpes simplex virus type 2 induces apoptosis in A549 cells through TP53I3 protein Authors - Yang Wang View Article
human	WB	Oncol Lett Prognostic prediction signature and molecular subtype for liver cancer: A CTC/CTM‑related gene prediction model and independent external validation Authors - Ling Xu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 14828-1-AP

WB analysis of HepG2 using 14828-1-AP

WB analysis of SMMC-7721 using 14828-1-AP

WB analysis of MCF-7 using 14828-1-AP

WB analysis of HeLa using 14828-1-AP

IP experiment of MCF-7 using 14828-1-AP

IHC staining of human liver cancer using 14828-1-AP