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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TP53I13 Polyclonal antibody (16596-1-AP)

    WB analysis using 16596-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16596-1-AP (TP53I13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using TP53I13 Polyclonal antibody (16596-1-AP)

    IHC staining of human heart using 16596-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16596-1-AP (TP53I13 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using TP53I13 Polyclonal antibody (16596-1-AP)

    IHC staining of human heart using 16596-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16596-1-AP (TP53I13 Antibody) at dilution of 1:50 (under 40x lens).

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TP53I13 Polyclonal antibody

TP53I13 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16596-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

DSCP1, TP53I13

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16596-1-AP targets TP53I13 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TP53I13 fusion protein Ag9746 相同性解析による交差性が予測される生物種
Full Name tumor protein p53 inducible protein 13
Calculated molecular weight 393 aa, 42 kDa
Observed molecular weight55 kDa
GenBank accession numberBC001593
Gene Symbol TP53I13
Gene ID (NCBI) 90313
RRIDAB_3085499
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NBR0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TP53I13 is involved in several processes, including negative regulation of cell cycle, response to UV, and response to xenobiotic stimulus.It may act as a tumor suppressorwhich could inhibits tumor cell growth, when overexpressed.

Protocols

Product Specific Protocols
WB protocol for TP53I13 antibody 16596-1-APDownload protocol
IHC protocol for TP53I13 antibody 16596-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols