Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse spleen tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28338-1-AP targets TOX3 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28251 Product name: Recombinant human TOX3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-167 aa of NM_001080430 Sequence: MDVRFYPAAAGDPASLDFAQCLGYYGYSKFGNNNNYMNMAEANNAFFAASEQTFHTPSLGDEEFEIPPITPPPESDPALGMPDVLLPFQALSDPLPSQGSEFTPQFPPQSLDLPSITISRNLVEQDGVLHSSGLHMDQSHTQVSQYRQDPSLIMRSIVHMTDAARSG 相同性解析による交差性が予測される生物種 |
| Full Name | TOX high mobility group box family member 3 |
| Calculated molecular weight | 63 aa |
| Observed molecular weight | 63 kDa |
| GenBank accession number | NM_001080430 |
| Gene Symbol | TOX3 |
| Gene ID (NCBI) | 27324 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15405 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TOX3, also known as TNRC9 (trinucleotide repeat containing 9), is a neuronal transcription factor containing a nuclear localization signal and a high mobility group (HMG)-box domain followed by a C-terminal poly-glutamine stretch. TOX3 is specifically expressed in the estrogen receptor alpha positive (ER+) subset of murine mammary luminal epithelial cells, including a recently identified progenitor cell subset (PMID: 19196971). TOX3 expression is negatively correlated with the immunotherapy targets PD-1, PD-L1 and HAVCR2 in lung adenocarcinoma, indicating that it serves a role in immunity activation (PMID: 27080130).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TOX3 antibody 28338-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |