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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A549 cells using TOX2 Recombinant antibody (84304-2-RR)

    IF Staining of A549 using 84304-2-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using TOX2 antibody (84304-2-RR, Clone: 241645A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using TOX2 Recombinant antibody (84304-2-RR)

    IF Staining of A549 using 84304-2-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using TOX2 antibody (84304-2-RR, Clone: 241645A11 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green).

  • Flow cytometry (FC) experiment of A549 cells using TOX2 Recombinant antibody (84304-2-RR)

    FC experiment of A549 using 84304-2-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Tox2 Recombinant Antibody (84304-2-RR, Clone:241645A11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Flow cytometry (FC) experiment of MCF-7 cells using TOX2 Recombinant antibody (84304-2-RR)

    FC experiment of MCF-7 using 84304-2-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Tox2 Recombinant Antibody (84304-2-RR, Clone:241645A11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 84304-2-RR

    Affinity and Kinetic Characterization of 84304-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84304-2-RR against Human TOX2 were performed. The affinity constant is 0.240 nM.

TOX2 Recombinant monoclonal antibody

TOX2 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 84304-2-RR
Clone No.241645A11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

GCX-1, GCX1, C20orf100, 241645A11

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inA549 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84304-2-RR targets TOX2 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag14772

Product name: Recombinant human TOX2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 303-488 aa of BC007636

Sequence: SSPDQGETKSTQANPPAKMLPPKQPMYAMPGLASFLTPSDLQAFRSGASPASLARTLGSKSLLPGLSASPPPPPSFPLSPTLHQQLSLPPHAQGALLSPPVSMSPAPQPPVLPTPMALQVQLAMSPSPPGPQDFPHISEFPSSSGSCSPGPSNPTSSGDWDSSYPSGECGISTCSLLPRDKSLYLT

相同性解析による交差性が予測される生物種
Full Name TOX high mobility group box family member 2
Calculated molecular weight 488 aa, 52 kDa
GenBank accession numberBC007636
Gene Symbol TOX2
Gene ID (NCBI) 84969
RRIDAB_3671844
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ96NM4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TOX2 is a transcription factor belonging to the TOX (thymocyte selection-associated HMG box) family that shares a highly conserved high mobility group DNA-binding domain with the other TOX members. A previous study showed that TOX2 is preferentially expressed in human NK cells among various leukocyte populations and is required for in vitro and in vivo human NK cell differentiation from UCB-derived CD341 hematopoietic stem cells (HSCs) (PMID: 25352127).

Protocols

Product Specific Protocols
IF protocol for TOX2 antibody 84304-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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