Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, MOLT-4 cells, Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
34301-1-AP targets TOX in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag41257 Product name: Recombinant human TOX protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 50-235 aa of BC016665 Sequence: DYVPASQSYPGPSLESEDFNIPPITPPSLPDHSLVHLNEVESGYHSLCHPMNHNGLLPFHPQNMDLPEITVSNMLGQDGTLLSNSISVMPDIRNPEGTQYSSHPQMAAMRPRGQPADIRQQPGMMPHGQLTTINQSQLSAQLGLNMGGSNVPHNSPSPPGSKSATPSPSSSVHEDEGDDTSKINGG 相同性解析による交差性が予測される生物種 |
| Full Name | thymocyte selection-associated high mobility group box |
| Calculated molecular weight | 526 aa, 58 kDa |
| Observed molecular weight | 57~70 kDa |
| GenBank accession number | BC016665 |
| Gene Symbol | TOX |
| Gene ID (NCBI) | 9760 |
| RRID | AB_3743187 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | O94900 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Thymocyte selection-associated high-mobility group box (TOX) is a DNA-binding factor that can regulate transcription by modifying local chromatin structure and modulating the formation of multi-protein complexes. TOX has multiple roles in the development of the adaptive immune system including development of CD4 T cells, NK cells and lymph node organogenesis (PMID: 35485425). Two bands detected at ~63 kDa and ~57kDa were presumed to be the unmodified form and the post-translationally form of TOX, respectively,(PMID: 32106280).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TOX antibody 34301-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |