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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using TOR3A Polyclonal antibody (10647-1-AP)

    WB analysis of HEK-293 using 10647-1-AP

    WB result of TOR3A antibody (10647-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOR3A transfected HEK-293 cells.

  • Western Blot (WB) analysis of HEK-293 cells using TOR3A Polyclonal antibody (10647-1-AP)

    WB analysis of HEK-293 using 10647-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10647-1-AP (TOR3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TOR3A Polyclonal antibody (10647-1-AP)

    WB analysis using 10647-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10647-1-AP (TOR3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using TOR3A Polyclonal antibody (10647-1-AP)

    WB analysis of HepG2 using 10647-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10647-1-AP (TOR3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using TOR3A Polyclonal antibody (10647-1-AP)

    IF Staining of HEK-293 using 10647-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 10647-1-AP (TOR3A antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO Validated

TOR3A Polyclonal antibody

TOR3A Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 10647-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

torsin family 3, member A, Torsin family 3 member A, Torsin 3A, ATP-dependent interferon-responsive protein, ADIR2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, Raji cells, PC-3 cells
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10647-1-AP targets TOR3A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1012

Product name: Recombinant human TOR3A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 97-397 aa of BC007571

Sequence: YLDLLTTWYCSFKDCCPRGDCRISNNFTGLEWDLNVRLHGQHLVQQLVLRTVRGYLETPQPEKALALSFHGWSGTGKNFVARMLVENLYRDGLMSDCVRMFIATFHFPHPKYVDLYKEQLMSQIRETQQLCHQTLFIFDEAEKLHPGLLEVLGPHLERRAPEGHRAESPWTIFLFLSNLRGDIINEVVLKLLKAGWSREEITMEHLEPHLQAEIVETIDNGFGHSRLVKENLIDYFIPFLPLEYRHVRLCARDAFLSQELLYKEETLDEIAQMMVYVPKEEQLFSSQGCKSISQRINYFLS

相同性解析による交差性が予測される生物種
Full Name torsin family 3, member A
Calculated molecular weight 46 kDa
Observed molecular weight 39-42 kDa, 46-52 kDa
GenBank accession numberBC007571
Gene Symbol TOR3A
Gene ID (NCBI) 64222
RRIDAB_2303598
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H497
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TOR3A antibody 10647-1-APDownload protocol
IF protocol for TOR3A antibody 10647-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols