Validation Data Gallery
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Tested Applications
| Positive WB detected in | COLO 320 cells, HeLa cells, HEK-293T cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IF | See 3 publications below |
| IP | See 1 publications below |
Product Information
21459-1-AP targets TOR1AIP1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14823 Product name: Recombinant human TOR1AIP1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-218 aa of BC023247 Sequence: MKTRRTTRLQQQHSEQPPLQPSPVTTRRGLRDSHSSEEDEASSQTDLSQTISKKTVRSIQEAPVSEDLVIRLRRPPLRYPRYEATSVQQKVNFSEEGETEEDDQDSSHSSVTTVKARSRDSDESGDKTTRSSSQYIESFWQSSQSQNFTAHDKQRSVLSSGYQKTPQEWAPQTARIRTRMQNDSILKSELGNQSPSTSSRQVTGQPQNASFVKRNRWW 相同性解析による交差性が予測される生物種 |
| Full Name | torsin A interacting protein 1 |
| Calculated molecular weight | 462aa,52 kDa; 583aa,66 kDa |
| Observed molecular weight | 50-55 kDa |
| GenBank accession number | BC023247 |
| Gene Symbol | TOR1AIP1 |
| Gene ID (NCBI) | 26092 |
| RRID | AB_2878861 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5JTV8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TOR1AIP1 antibody 21459-1-AP | Download protocol |
| WB protocol for TOR1AIP1 antibody 21459-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biochem Biophys Res Commun Analysis of the GFP-labelled β-dystroglycan interactome in HEK-293 transfected cells reveals novel intracellular networks | ||
Nat Cell Biol LAP1 supports nuclear adaptability during constrained melanoma cell migration and invasion |
