Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HeLa using 20549-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 20549-1-AP (TOP2B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 20549-1-AP
WB result of TOP2B antibody (20549-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOP2B transfected HepG2 cells.
WB analysis of HeLa using 20549-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 20549-1-AP (TOP2B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 20549-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 20549-1-AP (TOP2B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 20549-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 20549-1-AP (TOP2B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 20549-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 20549-1-AP (TOP2B antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 20549-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 20549-1-AP (TOP2B antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 20549-1-AP
WB result of TOP2B antibody (20549-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOP2B transfected HeLa cells.
WB analysis of HepG2 using 20549-1-AP
WB result of TOP2B antibody (20549-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOP2B transfected HepG2 cells.
IP experiment of HepG2 using 20549-1-AP
IP result of anti-TOP2B (IP:20549-1-AP, 4ug; Detection:20549-1-AP 1:300) with HepG2 cells lysate 3200ug.
IHC staining of human cervical cancer using 20549-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20549-1-AP (TOP2B antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human cervical cancer using 20549-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20549-1-AP (TOP2B antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human cervical cancer using 20549-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20549-1-AP (TOP2B antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human cervical cancer using 20549-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20549-1-AP (TOP2B antibody) at dilution of 1:100 (under 10x lens).
IF Staining of HeLa using 20549-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TOP2B antibody (20549-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 20549-1-AP
Immunofluorescent analysis of HepG2 cells, using TOP2B antibody 20549-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Featured Product
KD/KO Validated

TOP2B Polyclonal antibody

TOP2B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 20549-1-AP

Publications(17)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Synonyms

DNA topoisomerase 2 beta, TOP2B, top2beta, TOPIIB

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (17)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, HepG2 cells, K-562 cells
Positive IP detected in	HepG2 cells
Positive IHC detected in	human cervical cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 13 publications below
IHC	See 1 publications below
IF	See 5 publications below
IP	See 3 publications below
FC	See 1 publications below
ChIP	See 1 publications below

Product Information

20549-1-AP targets TOP2B in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	topoisomerase (DNA) II beta 180kDa
Calculated molecular weight	183 kDa
Observed molecular weight	183 kDa
GenBank accession number	NM_001068
Gene Symbol	TOP2B
Gene ID (NCBI)	7155
RRID	AB_10700004
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q02880
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TOP2B belongs to the type II topoisomerase family. TOP2B control topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2B makes double-strand breaks. It is indirectly ivolved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. The antibody is specific to TOP2B.

Protocols

Product Specific Protocols
IF protocol for TOP2B antibody 20549-1-AP	Download protocol
IHC protocol for TOP2B antibody 20549-1-AP	Download protocol
IP protocol for TOP2B antibody 20549-1-AP	Download protocol
WB protocol for TOP2B antibody 20549-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Science Chromosomes. A comprehensive Xist interactome reveals cohesin repulsion and an RNA-directed chromosome conformation. Authors - Anand Minajigi View Article
human,mouse	WB	Mol Cell The MYCN oncoprotein is an RNA-binding accessory factor of the nuclear exosome targeting complex Authors - Dimitrios Papadopoulos View Article
human	WB,IP	Mol Cell MYC assembles and stimulates topoisomerases 1 and 2 in a "topoisome". Authors - Subhendu K Das View Article
mouse	IF	Sci Adv XPF interacts with TOP2B for R-loop processing and DNA looping on actively transcribed genes Authors - Georgia Chatzinikolaou View Article
human,mouse	WB,IHC,IF,FC,IP	Cell Death Dis MDM2 provides TOP2 poison resistance by promoting proteolysis of TOP2βcc in a p53-independent manner Authors - Jianfeng Shu View Article
human	WB,ChIP	Cell Rep Topoisomerase IIα represses transcription by enforcing promoter-proximal pausing. Authors - Andrés Herrero-Ruiz View Article

Validation Data Gallery

WB analysis of HeLa using 20549-1-AP

WB analysis of HepG2 using 20549-1-AP

WB analysis of HeLa using 20549-1-AP

WB analysis of HepG2 using 20549-1-AP

WB analysis of HepG2 using 20549-1-AP

WB analysis of K-562 using 20549-1-AP

WB analysis of K-562 using 20549-1-AP

WB analysis of HeLa using 20549-1-AP

WB analysis of HepG2 using 20549-1-AP

IP experiment of HepG2 using 20549-1-AP

IHC staining of human cervical cancer using 20549-1-AP

IHC staining of human cervical cancer using 20549-1-AP

IHC staining of human cervical cancer using 20549-1-AP

IHC staining of human cervical cancer using 20549-1-AP

IF Staining of HeLa using 20549-1-AP

IF Staining of HepG2 using 20549-1-AP

TOP2B Polyclonal antibody

TOP2B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Chromosomes. A comprehensive Xist interactome reveals cohesin repulsion and an RNA-directed chromosome conformation.

The MYCN oncoprotein is an RNA-binding accessory factor of the nuclear exosome targeting complex

MYC assembles and stimulates topoisomerases 1 and 2 in a "topoisome".

XPF interacts with TOP2B for R-loop processing and DNA looping on actively transcribed genes

MDM2 provides TOP2 poison resistance by promoting proteolysis of TOP2βcc in a p53-independent manner

Topoisomerase IIα represses transcription by enforcing promoter-proximal pausing.

Able™

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