Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 18409-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18409-1-AP (TOMM40 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.
WB analysis using 18409-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18409-1-AP (TOMM40 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 18409-1-AP
IP result of anti-TOMM40 (IP:18409-1-AP, 4ug; Detection:18409-1-AP 1:1000) with mouse liver tissue lysate 4000ug.
IHC staining of human liver using 18409-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18409-1-AP (TOMM40 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 18409-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18409-1-AP (TOMM40 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 18409-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18409-1-AP (TOMM40 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 18409-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18409-1-AP (TOMM40 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 18409-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18409-1-AP (TOMM40 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 18409-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18409-1-AP (TOMM40 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 18409-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TOMM40 antibody (18409-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), (CL594-Phalloidin, red).
IF Staining of HeLa using 18409-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 18409-1-AP (TOMM40 antibody) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). F-actin is stained using CL555-phalloidin (red) and DNA is stained by DAPI (blue).
FC experiment of HepG2 using 18409-1-AP
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug TOMM40 Polyclonal antibody (18409-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

TOMM40 Polyclonal antibody

TOMM40 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 18409-1-AP

Publications(113)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

TOM40, C19orf1, D19S1177E, p38.5, PER EC1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (113)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, HepG2 cells, mouse kidney tissue, rat kidney tissue
Positive IP detected in	mouse liver tissue
Positive IHC detected in	human liver tissue, mouse heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, HeLa cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 94 publications below
IHC	See 1 publications below
IF	See 22 publications below
IP	See 2 publications below
CoIP	See 3 publications below

Product Information

18409-1-AP targets TOMM40 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, monkey, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag13065 Product name: Recombinant human TOMM40 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-361 aa of BC017224 Sequence: MGNVLAASSPPAGPPPPPAPALVGLPPPPPSPPGFTLPPLGGSLGAGTSTSRSSERTPGAATASASGAAEDGACGCLPNPGTFEECHRKCKELFPIQMEGVKLTVNKGLSNHFQVNHTVALSTIGESNYHFGVTYVGTKQLSPTEAFPVLVGDMDNSGSLNAQVIHQLGPGLRSKMAIQTQQSKFVNWQVDGEYRGSDFTAAVTLGNPDVLVGSGILVAHYLQSITPCLALGGELVYHRRPGEEGTVMSLAGKYTLNNWLATVTLGQAGMHATYYHKASDQLQVGVEFEASTRMQDTSVSFGYQLDLPKANLLFKGSVDSNWIVGATLEKKLPPLPLTLALGAFLNHRKNKFQCGFGLTIG 相同性解析による交差性が予測される生物種
Full Name	translocase of outer mitochondrial membrane 40 homolog (yeast)
Calculated molecular weight	38 kDa
Observed molecular weight	38 kDa
GenBank accession number	BC017224
Gene Symbol	TOMM40
Gene ID (NCBI)	10452
RRID	AB_2303725
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O96008
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The translocase of outer mitochondria membrane 40 (TOMM40, also known as TOM40), located in the center of the TOM complex, is a channel-forming subunit of translocase. It can facilitate the fluid movement of preproteins into the mitochondria by associating with TOMM20. TOMM40 plays a role in the assembly of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) by forming a complex with BCAP31 and mediating the translocation of Complex I components from the cytosol to the mitochondria (PMID: 31206022). TOMM40 has been reported to be associated with late-onset neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease.

Protocols

Product Specific Protocols
IF protocol for TOMM40 antibody 18409-1-AP	Download protocol
IHC protocol for TOMM40 antibody 18409-1-AP	Download protocol
IP protocol for TOMM40 antibody 18409-1-AP	Download protocol
WB protocol for TOMM40 antibody 18409-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Nature The ADP/ATP translocase drives mitophagy independent of nucleotide exchange. Authors - Atsushi Hoshino View Article
human	WB	Nature In vivo imaging of mitochondrial membrane potential in non-small-cell lung cancer. Authors - Milica Momcilovic View Article
human	IF	Cell Tau interactome maps synaptic and mitochondrial processes associated with neurodegeneration. Authors - Tara E Tracy View Article
human	WB	Cell Discov The Fe-S cluster assembly protein IscU2 increases α-ketoglutarate catabolism and DNA 5mC to promote tumor growth Authors - Xiaojun Ren View Article
human	WB	Cell Metab Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context. Authors - Marcel Morgenstern KD Validated View Article
monkey	WB,IF	Nat Cell Biol MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control. Authors - Tim König View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 18409-1-AP

WB analysis using 18409-1-AP

IP experiment of mouse liver using 18409-1-AP

IHC staining of human liver using 18409-1-AP

IHC staining of human liver using 18409-1-AP

IHC staining of mouse heart using 18409-1-AP

IHC staining of human liver using 18409-1-AP

IHC staining of human liver using 18409-1-AP

IHC staining of mouse heart using 18409-1-AP

IF Staining of HepG2 using 18409-1-AP

IF Staining of HeLa using 18409-1-AP

FC experiment of HepG2 using 18409-1-AP

TOMM40 Polyclonal antibody

TOMM40 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The ADP/ATP translocase drives mitophagy independent of nucleotide exchange.

In vivo imaging of mitochondrial membrane potential in non-small-cell lung cancer.

Tau interactome maps synaptic and mitochondrial processes associated with neurodegeneration.

The Fe-S cluster assembly protein IscU2 increases α-ketoglutarate catabolism and DNA 5mC to promote tumor growth

Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.

MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.