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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TOM1L2 Polyclonal antibody (32179-1-AP)

    WB analysis using 32179-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32179-1-AP (TOM1L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TOM1L2 Polyclonal antibody (32179-1-AP)

    WB analysis using 32179-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32179-1-AP (TOM1L2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using TOM1L2 Polyclonal antibody (32179-1-AP)

    WB analysis of mouse brain using 32179-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 32179-1-AP (TOM1L2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TOM1L2 Polyclonal antibody (32179-1-AP)

    IF Staining of HeLa using 32179-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TOM1L2 antibody (32179-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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TOM1L2 Polyclonal antibody

TOM1L2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 32179-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

TOM1-like protein 2, Target of Myb-like protein 2, Target Of Myb1 Like 2 Membrane Trafficking Protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, A-253 cells, BxPC-3 cells, HaCaT cells, LNCaP cells, U-251 cells, NIH/3T3 cells, rat brain tissue, mouse brain tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32179-1-AP targets TOM1L2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TOM1L2 fusion protein Ag37612 相同性解析による交差性が予測される生物種
Full Name target of myb1-like 2 (chicken)
Calculated molecular weight56 kDa，507aa
Observed molecular weight60 kDa
GenBank accession numberNM_001082968.1
Gene Symbol TOM1L2
Gene ID (NCBI) 146691
RRIDAB_3670215
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ6ZVM7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Target of Myb-like protein 2 (TOM1L2) acts as a MYO6/Myosin VI adapter protein that targets myosin VI to endocytic structures (PMID: 23023224). It may play a role in recruiting clathrin to endosomes and regulating growth factor-induced mitogenic signaling (PMID: 16412388; 16479011). TOM1L2 also regulates membrane trafficking that is linked to immunity and cell proliferation (PMID: 20604899).

Protocols

Product Specific Protocols
WB protocol for TOM1L2 antibody 32179-1-APDownload protocol
IF protocol for TOM1L2 antibody 32179-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols