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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TOLLIP Recombinant antibody (84711-5-RR)

    WB analysis using 84711-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84711-5-RR (TOLLIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TOLLIP Recombinant antibody (84711-5-RR)

    IHC staining of mouse brain using 84711-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84711-5-RR (TOLLIP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TOLLIP Recombinant antibody (84711-5-RR)

    IF Staining of HepG2 using 84711-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TOLLIP antibody (84711-5-RR, Clone: 242098E3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of U2OS cells using TOLLIP Recombinant antibody (84711-5-RR)

    FC experiment of U2OS using 84711-5-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug TOLLIP Recombinant antibody (84711-5-RR, Clone:242098E3) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84711-5-RR

    Affinity and Kinetic Characterization of 84711-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84711-5-RR against Human TOLLIP were performed. The affinity constant is 0.309 nM.

TOLLIP Recombinant monoclonal antibody

TOLLIP Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 84711-5-RR
Clone No.242098E3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

242098E3, IL 1RAcPIP, toll interacting protein, Toll-interacting protein

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84711-5-RR targets TOLLIP in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag1864

Product name: Recombinant human TOLLIP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-274 aa of BC012057

Sequence: MATTVSTQRGPVYIGELPQDFLRITPTQQQRQVQLDAQAAQQLQYGGAVGTVGRLNITVVQAKLAKNYGMTRMDPYCRLRLGYAVYETPTAHNGAKNPRWNKVIHCTVPPGVDSFYLEIFDERAFSMDDRIAWTHITIPESLRQGKVEDKWYSLSGRQGDDKEGMINLVMSYALLPAAMVMPPQPVVLMPTVYQQGVGYVPITGMPAVCSPGMVPVALPPAAVNAQPRCSEEDLKAIQDMFPNMDQEVIRSVLEAQRGNKDAAINSLLQMGEEP

相同性解析による交差性が予測される生物種
Full Name toll interacting protein
Calculated molecular weight 30 kDa
Observed molecular weight30 kDa
GenBank accession numberBC012057
Gene Symbol TOLLIP
Gene ID (NCBI) 54472
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9H0E2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TOLLIP (Toll interacting protein), is an inhibitory adaptor protein. Tollip is a negative regulator of IL-1R and Toll-like receptors signalling pathway, involved in inflammatory immune response and inhibiting IRAK1 phosphorylation and kinase activity (PMID:11441107, PMID:10854325, PMID:11751856). The TOLLIP-dependent selective autophagy pathway plays a role in clearance of cytotoxic polyQ proteins aggregates (PubMed:25042851).

Protocols

Product Specific Protocols
WB protocol for TOLLIP antibody 84711-5-RRDownload protocol
IHC protocol for TOLLIP antibody 84711-5-RRDownload protocol
IF protocol for TOLLIP antibody 84711-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols