Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of PC-3 using 11315-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 11315-1-AP (TOLLIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 11315-1-AP
WB result of TOLLIP antibody (11315-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOLLIP transfected HepG2 cells.
WB analysis using 11315-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11315-1-AP (TOLLIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 11315-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 11315-1-AP (TOLLIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11315-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11315-1-AP (TOLLIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 11315-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 11315-1-AP (TOLLIP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 11315-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 11315-1-AP (TOLLIP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human gliomas using 11315-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11315-1-AP (TOLLIP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 11315-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11315-1-AP (TOLLIP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of U2OS using 11315-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using TOLLIP antibody (11315-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

Featured Product
KD/KO Validated

TOLLIP Polyclonal antibody

TOLLIP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 11315-1-AP

Publications(16)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Toll-interacting protein, toll interacting protein, IL 1RAcPIP

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (16)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HepG2 cells, HEK-293 cells, human liver tissue, mouse brain tissue, PC-3 cells, rat brain tissue
Positive IHC detected in	human gliomas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	U2OS cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 6 publications below
WB	See 15 publications below
IF	See 3 publications below
IP	See 1 publications below

Product Information

11315-1-AP targets TOLLIP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1864 Product name: Recombinant human TOLLIP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-274 aa of BC012057 Sequence: MATTVSTQRGPVYIGELPQDFLRITPTQQQRQVQLDAQAAQQLQYGGAVGTVGRLNITVVQAKLAKNYGMTRMDPYCRLRLGYAVYETPTAHNGAKNPRWNKVIHCTVPPGVDSFYLEIFDERAFSMDDRIAWTHITIPESLRQGKVEDKWYSLSGRQGDDKEGMINLVMSYALLPAAMVMPPQPVVLMPTVYQQGVGYVPITGMPAVCSPGMVPVALPPAAVNAQPRCSEEDLKAIQDMFPNMDQEVIRSVLEAQRGNKDAAINSLLQMGEEP 相同性解析による交差性が予測される生物種
Full Name	toll interacting protein
Calculated molecular weight	30 kDa
Observed molecular weight	30 kDa
GenBank accession number	BC012057
Gene Symbol	TOLLIP
Gene ID (NCBI)	54472
RRID	AB_2256373
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H0E2
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TOLLIP (Toll interacting protein), is an inhibitory adaptor protein. Tollip is a negative regulator of IL-1R and Toll-like receptors signalling pathway, involved in inflammatory immune response and inhibiting IRAK1 phosphorylation and kinase activity (PMID:11441107, PMID:10854325, PMID:11751856). The TOLLIP-dependent selective autophagy pathway plays a role in clearance of cytotoxic polyQ proteins aggregates (PubMed:25042851).

Protocols

Product Specific Protocols
WB protocol for TOLLIP antibody 11315-1-AP	Download protocol
IHC protocol for TOLLIP antibody 11315-1-AP	Download protocol
IF protocol for TOLLIP antibody 11315-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Autophagy TOLLIP-mediated autophagic degradation pathway links the VCP-TMEM63A-DERL1 signaling axis to triple-negative breast cancer progression Authors - Tai-Mei Zhang View Article
human	WB	PLoS Pathog The MGF300-2R protein of African swine fever virus is associated with viral pathogenicity by promoting the autophagic degradation of IKKα and IKKβ through the recruitment of TOLLIP Authors - Tao Wang View Article
pig	WB	J Virol African Swine Fever Virus L83L Negatively Regulates the cGAS-STING-Mediated IFN-I Pathway by Recruiting Tollip To Promote STING Autophagic Degradation Authors - Mingyang Cheng View Article
human	WB	mBio The Globular C1q Receptor Is Required for Epidermal Growth Factor Receptor Signaling during Candida albicans Infection. Authors - Quynh T Phan KD Validated View Article
human	WB	Nat Commun Suppression of ACE2 SUMOylation protects against SARS-CoV-2 infection through TOLLIP-mediated selective autophagy Authors - Shouheng Jin KD Validated View Article
rat	IF	Gut Microbes Lactiplantibacillus plantarum 299v supplementation modulates β-cell ER stress and antioxidative defense pathways and prevents type 1 diabetes in gluten-free BioBreeding rats Authors - Pinar Sargin View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of PC-3 using 11315-1-AP

WB analysis of HepG2 using 11315-1-AP

WB analysis using 11315-1-AP

WB analysis of human liver using 11315-1-AP

WB analysis of HEK-293 using 11315-1-AP

WB analysis of rat brain using 11315-1-AP

WB analysis of mouse brain using 11315-1-AP

IHC staining of human gliomas using 11315-1-AP

IHC staining of human gliomas using 11315-1-AP

IF Staining of U2OS using 11315-1-AP

TOLLIP Polyclonal antibody

TOLLIP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

TOLLIP-mediated autophagic degradation pathway links the VCP-TMEM63A-DERL1 signaling axis to triple-negative breast cancer progression

The MGF300-2R protein of African swine fever virus is associated with viral pathogenicity by promoting the autophagic degradation of IKKα and IKKβ through the recruitment of TOLLIP

African Swine Fever Virus L83L Negatively Regulates the cGAS-STING-Mediated IFN-I Pathway by Recruiting Tollip To Promote STING Autophagic Degradation

The Globular C1q Receptor Is Required for Epidermal Growth Factor Receptor Signaling during Candida albicans Infection.

Suppression of ACE2 SUMOylation protects against SARS-CoV-2 infection through TOLLIP-mediated selective autophagy

Lactiplantibacillus plantarum 299v supplementation modulates β-cell ER stress and antioxidative defense pathways and prevents type 1 diabetes in gluten-free BioBreeding rats