Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| IHC | See 1 publications below |
Product Information
21564-1-AP targets TOAG1 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TOAG1 fusion protein Ag16067 相同性解析による交差性が予測される生物種 |
| Full Name | chromosome 3 open reading frame 23 |
| Calculated molecular weight | 496 aa, 58 kDa |
| Observed molecular weight | 35-41 kDa |
| GenBank accession number | BC101060 |
| Gene Symbol | TOAG1 |
| Gene ID (NCBI) | 285343 |
| RRID | AB_2878885 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N3R3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TOAG1, also named as TCAIM and C3orf23, is a mitochondrial protein which regulates T-cell apoptosis. It is a specific and reproducible marker for quiescence. TOAG1 has two isoforms with MW 41 kDa and 58 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TOAG1 antibody 21564-1-AP | Download protocol |
| IHC protocol for TOAG1 antibody 21564-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
FEBS J A novel role of TCAIM: suppressing renal carcinoma growth and enhancing its sensitivity to sunitinib
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