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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using TNRC4 Polyclonal antibody (25353-1-AP)

    WB analysis of mouse lung using 25353-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 25353-1-AP (TNRC4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using TNRC4 Polyclonal antibody (25353-1-AP)

    WB analysis of mouse spleen using 25353-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 25353-1-AP (TNRC4 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using TNRC4 Polyclonal antibody (25353-1-AP)

    IHC staining of human cerebellum using 25353-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 25353-1-AP (TNRC4 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using TNRC4 Polyclonal antibody (25353-1-AP)

    IHC staining of human testis using 25353-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25353-1-AP (TNRC4 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using TNRC4 Polyclonal antibody (25353-1-AP)

    IHC staining of human testis using 25353-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25353-1-AP (TNRC4 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using TNRC4 Polyclonal antibody (25353-1-AP)

    IHC staining of human cerebellum using 25353-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 25353-1-AP (TNRC4 Antibody) at dilution of 1:50 (under 10x lens).

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TNRC4 Polyclonal antibody

TNRC4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25353-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Bruno like protein 1, BRUNOL1, CAG repeat protein 4, CAGH4, CELF 3, CELF3, ERDA4, ETR 1, RNA binding protein BRUNOL 1, TNRC4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse lung tissue, mouse spleen tissue
Positive IHC detected inhuman testis tissue, human cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25353-1-AP targets TNRC4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TNRC4 fusion protein Ag18601 相同性解析による交差性が予測される生物種
Full Name trinucleotide repeat containing 4
Calculated molecular weight 464 aa, 51 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC052491
Gene Symbol TNRC4
Gene ID (NCBI) 11189
RRIDAB_2880041
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5SZQ8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TNRC4, also named as BRUNOL1, CAGH4, ERDA4, or TNRC4, is a 465 amino acid protein, which contains three RRM (RNA recognition motif) domains and belongs to the CELF/BRUNOL family. TNRC4 localizes in the nucleus and is expressed in brain. TNRC4 as a RNA-binding protein is involved in the regulation of pre-mRNA alternative splicing and mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing.

Protocols

Product Specific Protocols
WB protocol for TNRC4 antibody 25353-1-APDownload protocol
IHC protocol for TNRC4 antibody 25353-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols