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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TNNI1 Polyclonal antibody (22253-1-AP)

    WB analysis using 22253-1-AP (same clone as 22253-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22253-1-AP (TNNI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22253-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using TNNI1 Polyclonal antibody (22253-1-AP)

    IHC staining of human skeletal muscle using 22253-1-AP (same clone as 22253-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22253-1-AP (TNNI1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22253-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using TNNI1 Polyclonal antibody (22253-1-AP)

    IHC staining of human skeletal muscle using 22253-1-AP (same clone as 22253-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22253-1-AP (TNNI1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22253-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using TNNI1 Polyclonal antibody (22253-1-AP)

    IHC staining of human heart using 22253-1-AP (same clone as 22253-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 22253-1-AP (TNNI1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22253-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using TNNI1 Polyclonal antibody (22253-1-AP)

    IHC staining of human heart using 22253-1-AP (same clone as 22253-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 22253-1-AP (TNNI1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22253-1-PBS in a different storage buffer formulation.

TNNI1 Polyclonal antibody, PBS Only

TNNI1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 22253-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22253-1-PBS targets TNNI1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag17749

Product name: Recombinant human TNNI1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-63 aa of BC012600

Sequence: MPEVERKPKITASRKLLLKSLMLAKAKECWEQEHEEREAEKVRYLAERIPTLQTRGLSLSALQ

相同性解析による交差性が予測される生物種
Full Name troponin I type 1 (skeletal, slow)
Calculated molecular weight 187 aa, 22 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC012600
Gene Symbol TNNI1
Gene ID (NCBI) 7135
RRIDAB_11182276
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP19237
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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